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CXCL16 ELISA 试剂盒

CXCL16 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
产品编号 ABIN5652844
发货至: 中国
  • 抗原 See all CXCL16 ELISA试剂盒
    CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.625 ng/mL - 10 ng/mL
    最低检测浓度
    0.625 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Chemokine C-X-C-Motif Ligand 16 (CXCL16). No significant cross-reactivity or interference between Chemokine C-X-C-Motif Ligand 16 (CXCL16) and analogues was observed.
    灵敏度
    0.262 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Ligand 16 (CXCL16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Ligand 16 (CXCL16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Ligand 16 (CXCL16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Ligand 16 (CXCL16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X-C-Motif Ligand 16 (CXCL16) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X-C-Motif Ligand 16 (CXCL16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all CXCL16 ELISA试剂盒
    CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))
    别名
    Chemokine C-X-C-Motif Ligand 16 (CXCL16 产品)
    别名
    CXCL16 ELISA Kit, 0910001K24Rik ELISA Kit, AV290116 ELISA Kit, BB024863 ELISA Kit, CXCL16v1 ELISA Kit, CXCL16v2 ELISA Kit, SR-PSOX ELISA Kit, Zmynd15 ELISA Kit, b2b498Clo ELISA Kit, CXCLG16 ELISA Kit, SRPSOX ELISA Kit, C-X-C motif chemokine ligand 16 ELISA Kit, chemokine (C-X-C motif) ligand 16 ELISA Kit, CXCL16 ELISA Kit, Cxcl16 ELISA Kit
    背景

    Gene Name: Chemokine C-X-C-Motif Ligand 16

    Gene Aliases: SR-PSOX, CXCLG16, SRPSOX, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16

    基因ID
    58191
    UniProt
    Q9H2A7
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