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Transcription Factor EB (TFEB) ELISA 试剂盒

ABIN5526704 产品详细信息, 供应商: Log in to see
抗原
  • TFEB
  • tcfeb
  • MGC145796
  • alphatfeb
  • ALPHATFEB
  • BHLHE35
  • TCFEB
  • Tcfeb
  • bHLHe35
  • transcription factor EB
  • TFEB
  • tfeb
  • Tfeb
适用

实验类型
DNA-Binding ELISA
应用范围
ELISA
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原理 Human TFEB Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical TFEB binding sequences to semi-quantitatively detect active TFEB in lysates or nuclear extracts. Only available in North America.
样品类型 Cell Lysate, Nuclear Extract
Analytical Method Semi-Quantitative
检测方法 Colorimetric
特异性 The olionucleotide/antibody pair provided in this kit recognizes human TFEB in whole lysates and nuclear extracts.
产品特性
  • Specific transcription factor-DNA binding assay
  • Perfect alternative to EMSA
  • Easy to perform in an ELISA format
  • Non-radioactive assay
  • High throughput (96 well plate format)
  • Assay can be completed within 5 hours
组件
  • 96-well Strip Microplate pre-coated with DNA probes
  • DNA Binding Buffer
  • Positive Control Sample
  • Specific Competitor DNA probe
  • Non-specific Competitor DNA probe
  • Assay Reagent
  • DTT
  • Wash Buffer
  • Primary Antibody
  • HRP-conjugated Secondary Antibody
  • TMB One-Step Substrate Reagent
  • Stop Solution
试剂未包括
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Absorbent paper
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
别名 TFEB
基因ID 7942
UniProt P19484
研究领域 Transcription Factors, Chromatin and Nuclear Signaling
应用备注 Optimal working dilution should be determined by the investigator.
板类型 Pre-coated
实验流程
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared HRP-secondary antibody to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
限制 仅限研究用
储存条件 -20 °C
储存方法 Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
有效期 6 months