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RFP-Trap®

ABIN509416 产品详细信息, 供应商: Log in to see
抗原
适用
Discosoma
6
1
1
宿主
Camelidae
抗体类型
Recombinant Antibody
应用范围
Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif)
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原理 For biochemical analyses of RFP fusion proteins.
品牌 RFP-Trap®
样品类型 Cell Extracts
交叉反应 (详细) RFP-Trap efficiently pulls down various Red fluorescent proteins derived from DsRed, e.g. mRFP1, mCherry, mOrange, mPlum but also mRuby, mKate2 and RFP-tagged fusion proteins. No cross-reaction to DsRed, mRFPruby, TagRFP and all GFPs can be detected.
产品特性 Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. Due to their complex structure, the use of antibodies is often limited and hindered by batch-to-batch variations.

Camelidae (camels, dromedaries, llamas and alpacas) possess functional antibodies devoid of light chains, so-called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (~ 13 kDa).

Nano-Traps are based on single domain antibody fragments (VHHs) derived from alpaca.
纯化方法 Purified protein
组件 RFP-Trap® uncoupled
试剂未包括 Lysis buffer (CoIP), 10x RIPA buffer, Dilution buffer, Wash buffer, Elution buffer
别名 RFP
研究领域 Tags/Labels
应用备注 Red fluorescent proteins (RFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these RFP-fusion proteins and their interacting factors can be isolated fast and efficiently (one step) via immunoprecipitation using the RFP-Trap. The RFPTrap_A enables purification of any protein of interest fused to RFP.
实验时间 1.5 h
实验流程
  • Robust and versatile tool for biochemical analyses of RFP-fusion proteins
  • Short incubation times (5 - 30 min)
  • Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
  • Low unspecifi c binding
  • No contaminating heavy and light chains of conventional antibodies
  • Applicable in Chromatin Immunoprecipitation (ChIP)
限制 仅限研究用
状态 Liquid
浓度 250 µL resin (1mg/mL)
缓冲液 1 x PBS,0.01% Sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C
有效期 12 months
厂商提供的图像
 image for RFP-Trap® (ABIN509416) RFP-Trap A
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