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CA 19-9 CLIA Kit

CA 19-9 适用: 人 Chemiluminescent Sandwich ELISA
产品编号 ABIN504788
发货至: 中国
  • 抗原 See all CA 19-9 CLIA Kits
    CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))
    适用
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    检测方法
    Chemiluminescent
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Immunoenzymometric sequential assay (TYPE 4): The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies (enzyme and immobilized), with different and distinct epitope recognition, in excess, and native antigen. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-CA19-9 antibody. Upon mixing monoclonal biotinylated antibody, and a serum containing the native antigen, reaction results between the native antigen and the antibody, forming an antibody-antigen complex.
    Analytical Method
    Quantitative
    产品特性
    The Quantitative Determination of Cancer Antigen (CA 19-9) Concentration in Human Serum by a Microplate Chemiluminescence Immunoassay .
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  • 应用备注
    All products that contain human serum have been found to be non-reactive for Hepatitis B Surface Antigen, HIV 1&2 and HCV Antibodies by FDA required tests. Since no known test can offer complete assurance that infectious agents are absent, all human serum products should be handled as potentially hazardous and capable of transmitting disease. Good laboratory procedures for handling blood products can be found in the Center for Disease Control / National Institute of Health, "Biosafety in Microbiological and Biomedical Laboratories," 2nd Edition, 1988, HHS Publication No. (CDC) 88-8395.
    样本量
    25 μL
    板类型
    Pre-coated
    实验流程

    Specimien Collection and Preparation:

    The specimens shall be blood, serum in type and the usual precautions in the collection of venipuncture samples should be observed. For accurate comparison to established normal values, a fasting morning serum sample should be obtained. The blood should be collected in a plain redtop venipuncture tube without additives or anti-coagulants. Allow the blood to clot for samples. Centrifuge the specimen to separate the serum from the cells. Samples may be refrigerated at 2_x001E_8oC for a maximum period of five (5) days. If the specimen(s) cannot be assayed within this time, the sample(s) may be stored at temperatures of _x001E_20oC for up to 30 days. Avoid repetitive freezing and thawing. When assayed in duplicate, 0.050ml of the specimen is required.

    Reagent Preparation:

    1. Wash Buffer Dilute contents of Wash Concentrate to 1000ml with distilled or deionized water in a suitable storage container. Store diluted buffer at room temperature 20-27(C. 2. Working Signal Reagent Solution - Store at 2 - 8(C. Determine the amount of reagent needed and prepare by mixing equal portions of Signal Reagent A and Signal Reagent B in a clean container. For example, add 1 ml of A and 1ml of B per two (2) eight well strips (A slight excess of solution is made). Discard the unused portion if not used within 36 hours after mixing. If complete utilization of the reagents is anticipated, within the above time constraint, pour the contents of Signal Reagent B into Signal Reagent A and label accordingly.

    Test Procedure:

    Before proceeding with the assay, bring all reagents, serum references and controls to room temperature (20 - 27(C). 1. Format the microplates wells for each serum reference, control and patient specimen to be assayed in duplicate. Replace any unused microwell strips back into the aluminum bag, seal and store at 2-8(C. 2. Pipette 0.025 ml (25 l) of the appropriate serum reference, control or specimen into the assigned well. 3. Add 0.100 ml (100l) of the biotinylated labeled antibody to each well. It is very important to dispense all reagents close to the bottom of the coated well. 4. Swirl the microplate gently for 20-30 seconds to mix and cover. 5. Incubate 30 minutes at room temperature. 6. Discard the contents of the microplate by decantation or aspiration. If decanting, tap and blot the plate dry with absorbent paper. 7. Add 350l of wash buffer (see Reagent Preparation Section), decant (tap and blot) or aspirate. Repeat four (4) additional times for a total of five (5) washes. An automatic or manual plate washer can be used. Follow the manufacturers instruction for proper usage. If a squeeze bottle is employed, fill each well by depressing the container (avoiding air bubbles) to dispense the wash. Decant the wash and repeat four (4) additional times. 8. Add 0.100 ml (100l) of the Ca19-9 Tracer Reagent labeled antibody to each well. DO NOT SHAKE THE PLATE AFTER TRACER ADDITION 9. Cover and incubate 45 minutes at room temperature. 10. Discard the contents of the microplate by decantation or aspiration. If decanting, blot the plate dry with absorbent paper. 11. Add 350l of wash buffer (see Reagent Preparation Section), decant (tap and blot) or aspirate. Repeat four (4) additional times for a total of five (5) washes. An automatic or manual plate washer can be used. Follow the manufacturers instruction for proper usage. If a squeeze bottle is employed, fill each well by depressing the container (avoiding air bubbles) to dispense the wash. Decant the wash and repeat four (4) additional times. 12. Add 0.100 ml (100l) of working signal reagent to all wells (see Reagent Preparation Section). ). Always add reagents in the same order to minimize reaction time differences between wells. 13. Incubate for five (5) minutes in the dark. 14. Read the relative light units in each well for 0.2 1.0 seconds. The results should be read within thirty (30) minutes of adding the substrate solution.
    限制
    仅限研究用
  • 抗原 See all CA 19-9 CLIA Kits
    CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))
    别名
    CA 19-9 (CA 19-9 产品)
    背景
    A mucin type Sialyl Lewis Antigens group of glycoproteins (SLA) such as CA 19-9, 19-5 have been recognized as circulating cancer associated antigens for gastrointestinal cancer. The discovery of a monoclonal antibody clone (1116NS 19-9), which exhibited selective reactivity with human gastrointestinal carcinomas through the recognition of a carbohydrate determinant (CA 19-9) defined as a sialyl lacto-N-flucopenrose II, resulted in the successful purification and thus, determination of human gastrointestinal tumor associated glycoprotein antigen expressing CA 19-9 from colorectal carcinoma cell lines. Recently reports indicate that serum CA 19-9 level is frequently elevated in the circulation of patients with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Together with CEA elevated CA 19-9 is suggestive of gallbladder disease. The tumor associated antigen may also be associated in some malignant conditions. Research studies demonstrate that serum CA 19-9 values may have utility in monitoring subjects with the above mentioned diagnosed malignancies. In this method, CA19-9 calibrator, patient specimen or control is first added to a streptavidin coated well. Biotinylated monoclonal antibody (specific for CA19-9) is added and the reactants mixed. Reaction between the CA19-9 antibodies and native CA19-9 forms complex that binds with the streptavidin coated to the well. The excess serum proteins are washed away via a wash step. Another enzyme labeled monoclonal antibody specific to CA19-9 is added to the wells. The enzyme labeled antibody binds to the CA19-9 already immobilized on the well through its binding with the biotinylated monoclonal antibody. Excess enzyme is washed off via a wash step. Light is generated by the addition of a substrate. The intensity of the light generation is directly proportional to the concentration of the CA19-9 in the sample.
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