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PFKM ELISA 试剂盒

PFKM 适用: Cow Colorimetric Sandwich ELISA Plasma, Serum, Tissue Homogenate
产品编号 ABIN456135
发货至: 中国
  • 抗原 See all PFKM ELISA试剂盒
    PFKM (phosphofructokinase, Muscle (PFKM))
    适用
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Cow
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the specific measurement of bovine PFK concentrations in tissue homogenates, serum, and plasma.
    样品类型
    Tissue Homogenate, Serum, Plasma
    Analytical Method
    Quantitative
    特异性
    This assay recognizes recombinant and natural bovine PFK.
    交叉反应 (详细)
    No significant cross-reactivity or interference was observed.
    产品特性
    Bos taurus,Bovine,6-phosphofructokinase, muscle type,Phosphofructo-1-kinase isozyme A,PFK-A,Phosphofructokinase-M,Phosphofructokinase 1,Phosphohexokinase,PFKM,2.7.1.11
    组件
    Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A 1×120µl Detection Reagent B 1×120µl 2 Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
    Top Product
    Discover our top product PFKM ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PFK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PFK present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for PFK is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFK bound in the initial step. The color development is stopped and the intensity of the color is measured.
    试剂准备

    Bring all reagents to room temperature before use. Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate into deionized or distilled water to prepare 500 mL of Wash Buffer. Standard - Reconstitute the Standard with 1.0 mL of Sample Diluent. This reconstitution produces a stock solution of 100 U/mL. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard (100 U/mL). The Sample Diluent serves as the zero standard (0 U/mL). Detection Reagent A and B - Dilute to the working concentration specified on the vial label using Assay Diluent A and B (1:100), respectively.

    样品收集
    Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g. Remove the supernate and assay immediately or aliquot and store at ≤ -20 °C. Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C. Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 x g at 2 - 8 °C within 30 minutes of collection. Store samples at ≤ -20 °C. Avoid repeated freeze-thaw cycles. Note: Citrate plasma has not been validated for use in this assay.
    实验流程

    3 Allow all reagents to reach room temperature. Arrange and label required number of strips.
    1. Prepare all reagents, working standards and samples as directed in the previous sections.
    2. Add 100 uL of Standard, Control, or sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37 °C.
    3. Remove the liquid of each well, don’t wash.
    4. Add 100 uL of Detection Reagent A to each well. Incubate for 1 hour at 37°C. Detection Reagent A may appear cloudy. Warm to room temperature and mix gently until solution appears uniform.
    5. Aspirate each well and wash, repeating the process three times for a total of three washes. Wash by filling each well with Wash Buffer (350 uL) using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
    6. Add 100 uL of Detection Reagent B to each well. Cover with a new adhesive strip.Incubate for 1 hours at 37 °C.
    7. Repeat the aspiration/wash as in step
    5. 8. Add 90 uL of Substrate Solution to each well. Incubate for 30 minutes at room temperature. Protect from light.
    9. Add 50 uL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
    10. Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.
    Important Note:
    1. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
    2. It is recommended that no more than 32 wells be used for each assay run if manual pipetting is used since pipetting of all standards, specimens and controls should be completed within 5 minutes. A full plate of 96 wells may be used if automated pipetting is available.
    3. Duplication of all standards and specimens, although not required, is recommended.
    4. When mixing or reconstituting protein solutions, always avoid foaming.
    5. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
    6. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

    结果分析

    Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer 4 software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the PFK concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

    限制
    仅限研究用
  • 注意事项
    1. The kit should not be used beyond the expiration date on the kit label.
    2. Do not mix or substitute reagents with those from other lots or sources.
    3. If samples generate values higher than the highest standard, further dilute the samples with the Assay Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
    4. This assay is designed to eliminate interference by soluble receptors, ligands, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    储存条件
    4 °C/-20 °C
    储存方法
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • 抗原 See all PFKM ELISA试剂盒
    PFKM (phosphofructokinase, Muscle (PFKM))
    别名
    PFKM (PFKM 产品)
    别名
    GSD7 ELISA Kit, PFK-1 ELISA Kit, PFK1 ELISA Kit, PFKA ELISA Kit, PFKX ELISA Kit, AI131669 ELISA Kit, PFK-A ELISA Kit, PFK-M ELISA Kit, Pfk-4 ELISA Kit, Pfk4 ELISA Kit, Pfka ELISA Kit, Pfkx ELISA Kit, Pfk-M ELISA Kit, M-PFK ELISA Kit, PFKM ELISA Kit, pfkm ELISA Kit, zgc:92344 ELISA Kit, pfk ELISA Kit, im:7137110 ELISA Kit, wu:fc58g11 ELISA Kit, phosphofructokinase, muscle ELISA Kit, phosphofructokinase, platelet ELISA Kit, phosphofructokinase, muscle a ELISA Kit, phosphofructokinase, muscle S homeolog ELISA Kit, phosphofructokinase, muscle b ELISA Kit, PFKM ELISA Kit, Pfkm ELISA Kit, PFKP ELISA Kit, pfkma ELISA Kit, pfkm.S ELISA Kit, pfkmb ELISA Kit
    背景
    Phosphofructokinase (PFK) is a glycolytic enzyme that catalyzes the irreversible transfer of a phosphate from ATP to fructose-6-phosphate. PFK is the key regulatory enzyme for glycolysis. When ATP levels are high in the cell, the cell no longer needs metabolic energy production to occur. In this case, PFK's activity is inhibited by allosteric regulation by ATP itself, closing the valve on the flow of carbohydrates through glycolysis. Recall that allosteric regulators bind to a different site on the enzyme than the active (catalytic) site. Thus ATP binds in two places on PFK: in the active site as a substrate and in the regulatory site as a negative modulator. ATP bound in the regulatory site acts as a modulator by lowering the affinity of PFK for its other substrate, fructose-6-phosphate.
    途径
    Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect
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