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CaspGLOWTM Fluorescein Active Caspase-8 Staining Kit

ABIN412031 产品详细信息, 供应商: Log in to see
抗原
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8, apoptosis-related cysteine peptidase
  • death related ced-3/Nedd2-like protein
  • caspase-8
  • caspase 8
  • casp8
  • Dredd
  • CASP8
  • AaeL_AAEL014148
  • LOC100222284
  • Casp8
  • LOC100348477
适用
哺乳动物
26
15
15
4
4
2
1
1
1
1
1
1
1
应用范围
Detection (D), Fluorescence Microscopy (FM), Flow Cytometry (FACS)
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原理 The CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit provides a convenient means for sensitive detection of activated caspase-8 in living cells. The assay utilizes the caspase-8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a fluorescent in situ marker. FITC-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells.
品牌 CaspGLOW™
样品类型 Cell Samples
检测方法 Fluorometric
特异性 The CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit provides a convenient means for sensitive detection of activated caspase-8 in living cells. The assay utilizes the caspase-8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a fluorescent in situ marker. FITC-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells. The FITC label allows detection of activated caspase-8 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
产品特性 CaspGLOWTM Fluorescein Active Caspase-8 Staining Kit: Convenient & Sensitive Kit to Detect Activated Caspases-8 in Living Cells. Detection Method: Fluorescence Microscopy, Flow Cytometry or Fluorescence Plate Reader.
组件 FITC-IETD-FMK
Wash Buffer
Z-VAD-FMK
别名 Caspase-8 (CASP8 ELISA Kit 摘要)
背景 Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit provides a convenient means for sensitive detection of activated caspase-8 in living cells. The assay utilizes the caspase-8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a fluorescent in situ marker. FITC-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells.
研究领域 Apoptosis/Necrosis
应用备注 Sensitive detection of activated caspase-8 in living cells.
说明

Further details regarding sample type: Live cells

实验流程 A. Staining Procedure:
1. Induce apoptosis in cells (1 x 10^6 /mL) by desired method. Concurrently incubate a control culture without induction. An additional negative control can be prepared by adding the caspase inhibitor Z-VAD-FMK at 1 µL/mL to an induced culture to inhibit caspase activation.
2. Aliquot 300 µL each of the induced and control cultures into eppendorf tubes.
3. Add 1 µL of FITC-IETD-FMK into each tube and incubate for 0.5-1 hour at 37 °C incubator with 5 % CO 2.
4. Centrifuge cells at 3000 rpm for 5 minutes and remove supernatant.
5. Resuspend cells in 0.5 mL of Wash Buffer, and centrifuge again.
6. Repeat Step
5. Proceed to B, C, or D depending on methods of analysis. B. Quantification by Flow Cytometry: For flow cytometric analysis, resuspend cells in 300 µL of Wash buffer. Put samples on ice. Analyzing samples by flow cytometry using the FL-1 channel. C. Detection by Fluorescence Microscopy: For fluorescence microscopic analysis, resuspend cells in 100 µL Wash buffer. Add one drop of the cell suspension onto a microslide and cover with a coverslip. Observe cells under a fluorescence microscope using FITC filter. Caspase-8 positive cells appear to have brighter green signals, whereas caspase-8 negative control cells show much weaker signal. D. Analysis by Fluorescence Plate Reader: For analysis with fluorescence plate reader, resuspend cells in 100 µL Wash Buffer and then transfer the cell suspension to each well of the black microtiter plate. Measure the fluorescence intensity at Ex. = 485 nm and Em. = 535 nm. For control, use wells containing unlabeled cells.
限制 仅限研究用
储存条件 -20 °C
有效期 6-12 months
有引用在: Azad, Akter, Rogers, Nation, Velkov, Li: "Major pathways of polymyxin-induced apoptosis in rat kidney proximal tubular cells." in: Antimicrobial agents and chemotherapy, Vol. 59, Issue 4, pp. 2136-43, 2015 (PubMed).

Anginot, Espeli, Chasson, Mancini, Schiff: "Galectin 1 modulates plasma cell homeostasis and regulates the humoral immune response." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 11, pp. 5526-33, 2013 (PubMed).

Boulianne, Rojas, Haddad, Zaheen, Kapelnikov, Nguyen, Li, Hakem, Gommerman, Martin: "AID and caspase 8 shape the germinal center response through apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 12, pp. 5840-7, 2013 (PubMed).

Farias-de-Oliveira, Villa-Verde, Nunes Panzenhagen, Silva dos Santos, Berbert, Savino, de Meis: "Caspase-8 and caspase-9 mediate thymocyte apoptosis in Trypanosoma cruzi acutely infected mice." in: Journal of leukocyte biology, Vol. 93, Issue 2, pp. 227-34, 2013 (PubMed).

Marchi, Paoluzzi, Scotto, Seshan, Zain, Zinzani, OConnor: "Pralatrexate is synergistic with the proteasome inhibitor bortezomib in in vitro and in vivo models of T-cell lymphoid malignancies." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 14, pp. 3648-58, 2010 (PubMed).

Wang, Guo, Yang, Wang, Fu, Wang, Mao, Zhang, Li: "Cellular and molecular mechanisms of photodynamic hypericin therapy for nasopharyngeal carcinoma cells." in: The Journal of pharmacology and experimental therapeutics, Vol. 334, Issue 3, pp. 847-53, 2010 (PubMed).

Yang, Wang, Zhao, Zhu, Hou, Jun, Hou: "Contributions of TRAIL-mediated megakaryocyte apoptosis to impaired megakaryocyte and platelet production in immune thrombocytopenia." in: Blood, Vol. 116, Issue 20, pp. 4307-16, 2010 (PubMed).

Ito, Yip, Katz, Fonseca, Hedley, Chow, Xu, Wood, Bastianutto, Schimmer, Kelley, Liu: "Potential use of cetrimonium bromide as an apoptosis-promoting anticancer agent for head and neck cancer." in: Molecular pharmacology, Vol. 76, Issue 5, pp. 969-83, 2009 (PubMed).

Alajez, Mocanu, Shi, Chia, Breitbach, Hui, Knowles, Bell, Busson, Takada, Lo, OSullivan, Gullane, Liu: "Efficacy of systemically administered mutant vesicular stomatitis virus (VSVDelta51) combined with radiation for nasopharyngeal carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 15, pp. 4891-7, 2008 (PubMed).

Richter, Topham: "The alpha1beta1 integrin and TNF receptor II protect airway CD8+ effector T cells from apoptosis during influenza infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 8, pp. 5054-63, 2007 (PubMed).

Sharabi, Luger, Ben-David, Dayan, Zinger, Mozes: "The role of apoptosis in the ameliorating effects of a CDR1-based peptide on lupus manifestations in a mouse model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 8, pp. 4979-87, 2007 (PubMed).

Lin, Lee, Hung, Li, Yang-Yen, Yen: "Survival factor withdrawal-induced apoptosis of TF-1 cells involves a TRB2-Mcl-1 axis-dependent pathway." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 21962-72, 2007 (PubMed).

Wang, Müller, McPherson, Reichardt: "Glucocorticoids engage different signal transduction pathways to induce apoptosis in thymocytes and mature T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 3, pp. 1695-702, 2006 (PubMed).

Abdel-Latif, Murray, Renberg, ONeill, Porter, Jensen, Johnson: "Cell death in bovine parvovirus-infected embryonic bovine tracheal cells is mediated by necrosis rather than apoptosis." in: The Journal of general virology, Vol. 87, Issue Pt 9, pp. 2539-48, 2006 (PubMed).

Bahl, Kim, Calcagno, Ghersi, Puzone, Celada, Selin, Welsh: "IFN-induced attrition of CD8 T cells in the presence or absence of cognate antigen during the early stages of viral infections." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 7, pp. 4284-95, 2006 (PubMed).

Tzeng, Bolland, Inabe, Kurosaki, Pierce: "The B cell inhibitory Fc receptor triggers apoptosis by a novel c-Abl family kinase-dependent pathway." in: The Journal of biological chemistry, Vol. 280, Issue 42, pp. 35247-54, 2005 (PubMed).