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CaspGLOWTM Red Active Caspase Staining Kit

ABIN412023 产品详细信息, 供应商: Log in to see
抗原
  • CG5370
  • DCP-1
  • DCP1
  • Dcp1
  • Dmel\\CG5370
  • ccp1
  • dcp-1
  • dcp1
  • l(2)01862
  • l(2)02132
  • Death caspase-1
  • Dcp-1
适用
哺乳动物
2
1
应用范围
Detection (D), Fluorescence Microscopy (FM), Flow Cytometry (FACS)
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原理 The CaspGLOWTM Red Active Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
品牌 CaspGLOW™
样品类型 Cell Samples
检测方法 Fluorometric
特异性 The CaspGLOW™ Red Active Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells. The red fluorescence label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
产品特性 CaspGLOWTM Red Active Caspase Staining Kit: Convenient & Sensitive Kit to Detect Activated Caspases in Living Cells. Detection Method: Fluorescence Microscopy, Flow Cytometry or Fluorescence Plate Reader.
组件 Red-VAD-FMK
Wash Buffer
Z-VAD-FMK
别名 Caspase (CASP ELISA Kit 摘要)
背景 Activation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
应用备注 Detection of activated caspases in living cells.
说明

Further details regarding sample type: Live cells

实验流程 A. Staining Procedure:
1. Induce apoptosis in cells (1 x 10^6 /mL) by desired method. Concurrently incubate a control culture without induction. An additional control can be prepared by adding the caspase family inhibitor Z-VAD-FMK at 1 µL/mL to an induced culture to inhibit caspase activation.
2. Aliquot 300 µL each of the induced and control cultures into eppendorf tubes.
3. Add 1 µL of Red-VAD-FMK into each tube and incubate for 0.5-1 hour at 37 °C incubator with 5 % CO 2.
4. Centrifuge cells at 3000 rpm for 5 minutes and remove supernatant.
5. Resuspend cells in 0.5 mL of Wash Buffer, and centrifuge again.
6. Repeat Step
5. Proceed to B, C, or D depending on methods of analysis. B. Quantification by Flow Cytometry: For flow cytometric analysis, resuspend cells in 300 µL of Wash buffer. Put samples on ice. Analyzing samples by flow cytometry using the FL-2 channel (Ex. 540 nm, Em. = 570 nm). C. Detection by Fluorescence Microscopy: For fluorescence microscopic analysis, resuspend cells in 100 µL Wash buffer. Put one drop of the cell suspension onto a microslide and cover with a coverslip. Observe cells under a fluorescence microscope using rhodamine filter. Caspase positive cells appear to have brighter red signals, whereas caspase negative control cells show much weaker signal. D. Analysis by Fluorescence Plate Reader: For analysis with fluorescence plate reader, resuspend cells in 100 µL Wash Buffer and then transfer the cell suspension to each well of the black microtiter plate. Measure the fluorescence intensity at Ex/Em = 540/570 nm (Note: Ex/Em=488/570 nm will also work, although it's not an optimal wavelength). For control, use wells containing unlabeled cells.
限制 仅限研究用
储存条件 -20 °C
有效期 6-12 months
有引用在: Li, Xiong, Hu, Zhang: "Knockdown Epithelial Membrane Protein 1 Suppresses Human Degenerative Intervertebral Disc-Derived Nucleus Pulposus Cell Proliferation." in: Cartilage, Vol. 2, Issue 3, pp. 300-6, 2015 (PubMed).

He, Meyer-Hermann, Xiangying, Zuan, Jones, Ramakrishna, de Vries, Dolpady, Aina, Joseph, Narayanan, Subramaniam, Puthia, Wong, Xiong, Poidinger, Urban, Lafaille, Curotto de Lafaille: "The distinctive germinal center phase of IgE+ B lymphocytes limits their contribution to the classical memory response." in: The Journal of experimental medicine, Vol. 210, Issue 12, pp. 2755-71, 2013 (PubMed).

Moresi, Pristerà, Scicchitano, Molinaro, Teodori, Sassoon, Adamo, Coletti: "Tumor necrosis factor-alpha inhibition of skeletal muscle regeneration is mediated by a caspase-dependent stem cell response." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 4, pp. 997-1008, 2008 (PubMed).

Alajez, Mocanu, Shi, Chia, Breitbach, Hui, Knowles, Bell, Busson, Takada, Lo, OSullivan, Gullane, Liu: "Efficacy of systemically administered mutant vesicular stomatitis virus (VSVDelta51) combined with radiation for nasopharyngeal carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 15, pp. 4891-7, 2008 (PubMed).

Veschini, Belloni, Foglieni, Cangi, Ferrarini, Caligaris-Cappio, Ferrero: "Hypoxia-inducible transcription factor-1 alpha determines sensitivity of endothelial cells to the proteosome inhibitor bortezomib." in: Blood, Vol. 109, Issue 6, pp. 2565-70, 2007 (PubMed).

Abdel-Latif, Murray, Renberg, ONeill, Porter, Jensen, Johnson: "Cell death in bovine parvovirus-infected embryonic bovine tracheal cells is mediated by necrosis rather than apoptosis." in: The Journal of general virology, Vol. 87, Issue Pt 9, pp. 2539-48, 2006 (PubMed).