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Further details regarding sample type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media
Calculation: Correct background by subtracting the value derived from the 0 D-lactate control from all standard and sample readings (Background can be significant and must be subtracted from all readings). Plot a standard curve of nM/well vs. OD 450nm. Apply the sample readings to the standard curve. Calculate the D-Lactate concentrations of the test samples: C = La/Sv (nM/µL, µM/mL or mM) Where: La is the D-lactate amount (nmol) of your sample from the standard curve. Sv is the sample volume (µL) added into the well. D-Lactic acid molecular weight: 90.08