Thrombopoietin ELISA 试剂盒
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- 抗原 See all Thrombopoietin (THPO) ELISA试剂盒
- Thrombopoietin (THPO)
- 抗原表位
- AA 22-356
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TPO
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: S22-T356 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <5pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: S22-T356 - Featured
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Thrombopoietin (THPO) ELISA Kit
THPO 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thrombopoietin (THPO) ELISA KitTHPO 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thrombopoietin (THPO) ELISA KitTHPO 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Thrombopoietin (THPO) ELISA KitTHPO 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thrombopoietin (THPO) ELISA KitTHPO 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Thrombopoietin (THPO) ELISA KitTHPO 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Thrombopoietin (THPO) ELISA KitTHPO 适用: Cow Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Thrombopoietin (THPO) ELISA KitSmall Sample THPO 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thrombopoietin (THPO) ELISA KitTHPO 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Found mainly in the liver, kidney and skeletal muscle.
- 板类型
- Pre-coated
- 实验流程
- mouse TPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TPO has been precoated onto 96-well plates. Standards(NSO, S22-T356) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TPO amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse ell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TPO standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 296, Standard deviation: 11.84, CV(%): 4
- Sample 2: n=16, Mean(pg/ml): 611, Standard deviation: 33, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 1265, Standard deviation: 82.23, CV(%): 6.5,
- Sample 1: n=24, Mean(pg/ml): 321, Standard deviation: 16.7, CV(%): 5.2
- Sample 2: n=24, Mean(pg/ml): 746, Standard deviation: 47, CV(%): 6.3
- Sample 3: n=24, Mean(pg/ml): 1464, Standard deviation: 105.4, CV(%): 7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model." in: Frontiers in pharmacology, Vol. 7, pp. 154, (2016) (PubMed).
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: "Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model." in: Frontiers in pharmacology, Vol. 7, pp. 154, (2016) (PubMed).
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- 抗原 See all Thrombopoietin (THPO) ELISA试剂盒
- Thrombopoietin (THPO)
- 别名
- Thrombopoietin (TPO) (THPO 产品)
- 别名
- MGDF ELISA Kit, MKCSF ELISA Kit, ML ELISA Kit, MPLLG ELISA Kit, THCYT1 ELISA Kit, TPO ELISA Kit, Mgdf ELISA Kit, Ml ELISA Kit, Mpllg ELISA Kit, Tpo ELISA Kit, Tpo1 ELISA Kit, Tpo2 ELISA Kit, Tpo3 ELISA Kit, Tpo4 ELISA Kit, tpo ELISA Kit, LOC100231762 ELISA Kit, thrombopoietin ELISA Kit, THPO ELISA Kit, Thpo ELISA Kit, thpo ELISA Kit
- 背景
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Protein Function: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Background: Thrombopoietin(TPO) is also called megakaryocyte growth and development factor(MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.
Synonyms: Thrombopoietin,C-mpl ligand,ML,Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor,MGDF,Myeloproliferative leukemia virus oncogene ligand,Thpo,
Full Gene Name: Thrombopoietin
Cellular Localisation: Secreted. - 基因ID
- 21832
- UniProt
- P40226
- 途径
- JAK/STAT Signaling, Hormone Activity
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