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MMP8 ELISA 试剂盒

MMP8 适用: 人 AA 21-467 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum
产品编号 ABIN411332
发货至: 中国
  • 抗原 See all MMP8 ELISA试剂盒
    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
    抗原表位
    AA 21-467
    适用
    • 7
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    • 3
    • 1
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156-10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-8
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Saliva
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: F21-G467
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: F21-G467
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Belongs to the peptidase M10A family.

    Tissue Specificity: Neutrophils.

    板类型
    Pre-coated
    实验流程
    human MMP-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-8 has been precoated onto 96-well plates. Standards(NSO, F21-G467) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-8 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-8 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 1275, Standard deviation: 56.1, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 3563, Standard deviation: 185.3, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 6822, Standard deviation: 395.7, CV(%): 5.8,
    • Sample 1: n=24, Mean(pg/ml): 1370, Standard deviation: 69.87, CV(%): 5.1
    • Sample 2: n=24, Mean(pg/ml): 3728, Standard deviation: 242.32, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 7142, Standard deviation: 521.4, CV(%): 7.3
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Kumar, Jain, Chauhan, Bharate, Koli, Kumar: "Influence of different forms and materials (zirconia or titanium) of abutments in periimplant soft-tissue healing using matrix metalloproteinase-8: A randomized pilot study." in: The Journal of prosthetic dentistry, (2017) (PubMed).

    Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

    Akbari, Prabhuji, Karthikeyan, Raghunatha, Narayanan: "Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test." in: Journal of Indian Society of Periodontology, Vol. 19, Issue 5, pp. 525-30, (2015) (PubMed).

  • 抗原 See all MMP8 ELISA试剂盒
    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
    别名
    MMP8 (MMP8 产品)
    别名
    MMP8 ELISA Kit, BB138268 ELISA Kit, CLG1 ELISA Kit, HNC ELISA Kit, MMP-8 ELISA Kit, PMNL-CL ELISA Kit, matrix metallopeptidase 8 ELISA Kit, neutrophil collagenase ELISA Kit, MMP8 ELISA Kit, Mmp8 ELISA Kit, LOC100339790 ELISA Kit
    背景

    Protein Function: Can degrade fibrillar type I, II, and III collagens.

    Background: Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

    Synonyms: Neutrophil collagenase,3.4.24.34,Matrix metalloproteinase-8,MMP-8,PMNL collagenase,PMNL-CL,MMP8,CLG1,

    Full Gene Name: Neutrophil collagenase

    Cellular Localisation: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.
    基因ID
    4317
    UniProt
    P22894
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