Nitrite/Nitrate Determination Assay Kit

ABIN3172710 产品详细信息, 供应商: Log in to see
检测范围
1-100 μM
最低检测浓度
1 μM
应用范围
Biochemical Assay (BCA)
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原理 The kit is designed for nitrite/nitrate determination in biological samples.
样品类型 Plasma
检测方法 Colorimetric
特异性 Specific for nitrite/nitrate determination in plasma samples.
产品特性 The overproduction of nitric oxide may lead to oxidative and nitrosative stress. It has been demonstrated that they enhance the development of a variety of diseases, as well as the ageing process1. Regarding nitrosative stress, high levels of iNOS have be
组件 Plate and the reagents neccesary to perform the assay.
试剂未包括 Pipettes, reaction tubes, plate reader.
说明

For nitrite only: 50μL, For Nitrite/nitrate: 100μL

实验时间 2 h
板类型 Uncoated
试剂准备

Reagent Preparation: 1. To prepare Reagent A solution: Add exactly 1000 μL of ultrapure water to Reagent A and mix thoroughly. This reagent must be freshly prepared. 2. To prepare Reagent C solution: Add exactly 1000 μL of ultrapure water to Reagent C and mix thoroughly. This reagent must be freshly prepared.

样品制备

It is recommended to assay the samples in duplicate. Plasma samples may be deproteinized before performing the assay.

实验流程

Standard Preparation: Prepare the calibrate in 1 mL tubes following the Table 1 (see kit booklet). Use ultrapure water as diluent. Performing the assay: The following procedure is for the determination of nitrite + nitrate: 1. Add 50 μL of the sample or standard in each well (96-well plate). 2.Add 10 μL of Reagent A and 20 μL of Reagent B. Incubate for 60 minutes. 3.Add 10 μL of Reagent C and 10 μL of Reagent D. Incubate for 20 minutes. 4.Add 50 μL of Reagent E in each well. Incubate for 10 minutes protected from light. 5.Add 50 μL of Reagent F in each well. Incubate for 10 minutes protected from light. 6.Read the absorbance at 540 nm within 30 minutes. In order to measure only the nitrite in the sample (not both nitrite and nitrate, "total nitrite"), add ultrapure water instead of Reagents A, B, C and D and continue the assay by adding Reagents E and F as shown in the procedure.

结果分析

1.Calculate the average absorbance of each sample and control. 2.Determine the concentration in the sample by comparison to the Nitrite Standard reference curve (Figure 4 see Images). Nitrite (μM) = (?A540nm- intercept)/ slope

限制 仅限研究用
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
厂商提供的图像
Biochemical Assay (BCA) image for Nitrite/Nitrate Determination Assay Kit (ABIN3172710) Typical standard curve