Nitrite Determination Assay Kit

ABIN3172709 产品详细信息, 供应商: Log in to see
检测范围
1-100 μM
最低检测浓度
1 μM
应用范围
Biochemical Assay (BCA)
选项
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原理 The kit is designed for nitrite determination in biological samples.
样品类型 Plasma
检测方法 Colorimetric
特异性 Specific for nitrite determination in plasma samples.
产品特性 The overproduction of nitric oxide may lead to oxidative and nitrosative stress. It has been demonstrated that they enhance the development of a variety of diseases, as well as the ageing process1. Regarding nitrosative stress, high levels of iNOS have be
组件 Plate and the reagents neccesary to perform the assay.
试剂未包括 Pipettes, reaction tubes, plate reader.
样本量 50 μL
实验时间 1 h
板类型 Uncoated
试剂准备

No reagent preparation is needed.

样品制备

Plasma samples may be deproteinized before performing the assay.

实验流程

Standard Preparation: Prepare the calibrate in 1 mL tubes following the Table 1 ( see in booklet) . Use ultrapure water as diluent. Performing the assay: 1. Add 50 μL of samples or standards in each well (96-well plate). 2.Add 50 μL of Reagent A in each well. Incubate for 10 minutes protected from light. 3.Add 50 μL of Reagent B in each well. Incubate for 10 minutes protected from light. 4.Read the absorbance at 540 nm within 30 minutes.

结果分析

Determine average absorbance value of each experimental sample. Determine its concentration by comparison to the Nitrite Standard reference curve (Figure 2 see images). Nitrite (μM) = (?A540nm- intercept)/ slope

限制 仅限研究用
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
厂商提供的图像
Biochemical Assay (BCA) image for Nitrite Determination Assay Kit (ABIN3172709) Typical standard curve