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FRAP Antioxidant Capacity Assay Kit

ABIN3172706 产品详细信息, 供应商: Log in to see
检测范围
7.5-250 μg/mL
最低检测浓度
7.5 μg/mL
应用范围
Biochemical Assay (BCA)
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原理 The kit is designed for the antioxidant capacity measurement in biological samples.
样品类型 Biological Fluids
检测方法 Colorimetric
特异性 Specific for antioxidant activity determination.
产品特性 FRAP assay kit is recommended for total antioxidant activity of single antioxidants in aqueous solution and added to plasma. The assay described here measures the ferric reducing ability of plasma (FRAP). At low pH , when a ferric complex is red
组件 Plate and the reagents neccesary to perform the assay.
试剂未包括 Pipettes, reaction tubes, plate reader.
样本量 10 μL
实验时间 15 min
板类型 Uncoated
试剂准备

Reagents Preparation: 1. Add exactly 3.5 mL of ultrapure water in each vial of Reagent B and mix thoroughly. Once dissolved, keep refrigerated at -20 °C. (solution B). 2. Prepare FRAP working solution just before use by mixing Reagents A, B and C (10:1:1). 3. FRAP standard: Add exactly 1 mL of ultrapure water in each Standard vial and mix thoroughly. Prepare standards immediately prior to the assay performed. Do not store the standard preparations.

样品制备

Sample Preparation: Dilute your sample to an absorbance value corresponding to 500-600 μM of standard approximately. Plasma samples do not usually need to be diluted.

实验流程

Standard Preparation Antioxidant activity is expressed as FRAP values (Ferric Reducing Ability of Plasma). These values are related to Fe2+ concentration. Prepare calibration curve in 1 mL tubes as shown below in Table 1 (see kit booklet).Performing the assay: 1. Add 10 μL of the sample or standard in each well. 2.Add 220 μL of FRAP working solution previously prepared (see Reagents Preparation) in each well. Final dilution of the sample in the reaction mixture will be therefore, 1/22. 3.Mix the mixture for 4 minutes under continuous stirring. 4.Read the absorbance at 593 nm.

结果分析

1.Zeroed the absorbance values: ?A593nm = ?A593nm sample/standard - A593nm blank. Where A593nm sample/standard is the absorbance measured 4 minutes after the addition of antioxidants from samples or standards. 2.Plot the zeroed absorbance (?A593nm) of standards as a function of their final concentrations (Table 1). See Figure 2 for a typical standard curve (see images). 3.Calculate the FRAP value of the samples using the equation obtained from the linear regression of the standard curve substituted ?A593nmvalues for each sample. FRAP (μM) = (?A593nm- intercept)/ slope

限制 仅限研究用
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
厂商提供的图像
Biochemical Assay (BCA) image for FRAP Antioxidant Capacity Assay Kit (ABIN3172706) Typical standard curve