BCA Protein Quantification Assay Kit

ABIN3172699 产品详细信息, 供应商: Log in to see
抗原
检测范围
0.5-2000 μg/mL
最低检测浓度
0.5 μg/mL
应用范围
Biochemical Assay (BCA), Quantification (Q)
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
原理 The kit is designed for protein quantification in biological samples.
样品类型 Plasma, Tissue Homogenate, Urine, Serum, Cell Lysate
Analytical Method Quantitative
检测方法 Colorimetric
特异性 Specific for protein quantification.
产品特性 The BCA method1 combines the biuret reaction2 with the colorimetric detection of the monovalent copper ion by bicinchoninic acid (BCA). In this reaction, protein reduces Cu2+ to Cu+ in an alkaline environment .After the reduction of the divalent copper io
组件 Plate and the reagents neccesary to perform the assay.
试剂未包括 Pipettes, reaction tubes, plate reader, centrifuge.
别名 BCA
说明

75 ul (microassay) / 10μL (microplate)

实验时间 25 min
板类型 Uncoated
试剂准备

BCA Working Solution: Use the following formula to determine the total volume or Working Solution required:(standards + samples) x (replicates) x (volume of Working Solution per sample) = Total volume Working Solution Required 50:1 (v/v) Reagent A/ Reagent B. Prepare fresh daily

实验流程

MICROASSAY:1.Prepare the calibrate in 1.5 mL tubes following the Table 2 (see kit booklet). For the diluent, use the same buffer as in the samples. 2.Pipette 75 μL of each standard or unknown sample solution into separate 1.5 mL tubes.3. To each tube, add 1425 μL of freshly prepared BCA Working Solution. Incubate the tubes 15 min at 60 °C. 4.Cool all samples to room temperature.5. Mix the samples, zero the spectrophotometer with the blank and measure the absorbance at 562 nm.
MICROPLATE: 1.Prepare the calibrate in 1.5 mL tubes following the Table 3. For the diluent use the same buffer as in the samples. 2.Pipette 10 μL of each standard and unknown sample replicate into a microplate well. Refer to the Table 3 as a guide for diluting the protein standard (See kit booklet). For the diluent, use the same buffer as in the samples. 3.Add 200 μL of freshly prepared BCA Working Solution and immediately mix the microplate on plate mixer for 30 seconds. 4.Cover and incubate the microplate 15 min at 60 °C. 5. Cool plate to room temperature. 6.Mix the samples, zero the spectrophotometer with the blank and measure the absorbance at 562 nm.

结果分析

1.If the spectrophotometer or microplate reader was not zeroed with the blank, then average the blank values and substract the average blank value from the standard and unknown sample values. 2.Create a standard curve by plotting O.D. 562nm (y-axis) vs standard, μg (x-axis). Determine the unknown sample concentration using the standard curve. 3.The level of detection of the assay is lower for the microplate assay when compared with the microassay due to a shorter light path used in the microplate reader. 4.Standard curve example for microplate assay procedure is shown in Figure 3. (See Images) 5.Measure the absorbance of these standards, blanks and unknown samples at 562nm.

限制 仅限研究用
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
厂商提供的图像
Quantification (Q) image for BCA Protein Quantification Assay Kit (ABIN3172699) Typical standard curve
您还需要查找其他产品吗?