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Estriol ELISA 试剂盒

适用: 人 Colorimetric Competition ELISA 0.075-15 ng/mL Serum
产品编号 ABIN3073428
发货至: 中国
  • 抗原 See all Estriol ELISA试剂盒
    Estriol
    适用
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    0.075-15 ng/mL
    最低检测浓度
    0.075 ng/mL
    应用范围
    ELISA
    原理
    The Unconjugated Estriol Enzyme Immunoassay (EIA) Kit provides materials for the quantitative measurement of unconjugated estriol in serum. This assay is intended for Research Use only. The test may help aid in the diagnosis and treatment of fetoplacental distress.
    样品类型
    Serum
    Analytical Method
    Quantitative
    灵敏度
    0.044 ng/mL
    组件
    • Estriol Calibrators
    • Estriol Controls AG-002 Goat Anti-Rabbit IgG (GARG) Microtitration Strips
    • Estriol Biotin Conjugate Concentrate
    • Estriol Conjugate Diluent
    • Anti-Estriol Antibody Solution
    • Estriol Streptavidin Conjugate Ready-to-Use
    • TMB Chromogen Solution
    • Wash Concentrate A
    • Stopping Solution
    试剂未包括
    1. Microtitration plate reader capable of absorbance measurement at 450 nm, 405nm and 630 nm.
    2. Microtitration orbital plate shaker.
    3. Microtitration plate washer.
    4. Semi-automated/manual precision pipette to deliver 10-250 μL.
    5. Repeater pipette
    6. Repeater plus for repeated dispensing
    7. Vortex mixer.
    8. Deionized water.
    Top Product
    Discover our top product Estriol ELISA Kit
  • 样本量
    25 μL
    实验时间
    1 h
    板类型
    Pre-coated
    试剂准备
    1. Wash Solution: Dilute wash concentrate 25-fold with deionized water. The wash solution is stable for one month at room temperature when stored in a tightly sealed bottle.
    2. Microtitration Wells: Select the number of coated wells required for the assay. The remaining unused wells should be placed in the resealable pouch with a desiccant. The pouch must be resealed to protect from moisture.
    样品收集
    • Serum is the recommended sample type.
    • Sample handling, processing, and storage requirements depend on the brand of blood collection tube6 that you use. Please reference the manufacturer's instructions for guidance. Each laboratory should determine the acceptability of its own blood collection tubes and serum separation products.
    • Samples may be stored at 4 °C if assayed within 7 days, otherwise samples must be stored at -20 °C or -80 °C to avoid loss of bioactivity and contamination.
    • Avoid assaying lipemic, hemolyzed or icteric samples.
    • Avoid repeated freezing and thawing of samples. Thaw samples no more than 3 times.
    • For shipping, place specimens in leak proof containers in biohazard specimen bags with appropriate specimen identification and test requisition information in the outside pocket of the biohazard specimen bag. Follow DOT and IATA requirements when shipping specimens.
    实验流程

    Allow all specimens and reagents to reach room temperature (23 ± 2 °C) and mix thoroughly by gentle inversion before use. Calibrators, controls, and unknowns should be assayed in duplicate. NOTE: All serum samples reading higher than the highest calibrator should be mixed and diluted in the 0 ng/mL Calibrator A prior to assay.

    1. Prepare the Estriol-Biotin Conjugate Solution by diluting the Estriol Biotin Conjugate Concentrate in Estriol Conjugate Diluent as described under the Preparation of the Reagents section
    2. Label the microtitration strips to be used.
    3. Pipette 50 μL of the Calibrators and Controls to the appropriate wells.
    4. Pipette 25 μL of the Calibrator A to the remaining wells which will be used. Pipette 25 μL of Unknowns in the wells containing Calibrator A.
    5. Add 100 μL of Estriol-Biotin Conjugate Solution to each well using a repeater pipette.
    6. Add 100 μL of Estriol Antibody Solution to each well using a repeater pipette.
    7. Incubate the plate, shaking at a fast speed (600-800 rpm) on an orbital microplate shaker, for 30 minutes at room temperature (23 ± 2 °C).
    8. Aspirate and wash each strip 5 times with Washing Solution (350 μL/per well) using an automatic microplate washer.
    9. Add 100 μL of the Streptavidin-Enzyme Conjugate-RTU to each well using a repeater pipette.
    10. Incubate the plate, shaking at a fast speed (600-800 rpm) on an orbital microplate shaker, for 30 minutes at room temperature (23 ± 2 °C).
    11. Aspirate and wash each strip 5 times with the Wash Solution (350 μL/per well) using an automatic microplate washer.
    12. Add 100 μL of the TMB chromogen solution to each well using a repeater pipette. Avoid exposure to direct sunlight.
    13. Incubate the wells, shaking at 600-800 rpm on an orbital microplate shaker, for 10-12 min at room temperature (23 ± 2 °C). NOTE: Visually monitor the color development to optimize the incubation time.
    14. Add 100 μL of the Stopping solution to each well using a repeater pipette. Read the absorbance of the solution in the wells within 20 minutes, using a microplate reader set to 450 nm. NOTE: If instrument has a wavelength correction, set the instrument to dual wavelength measurement at 450 nm with background wavelength correction at 630 nm.

    结果分析
    1. Optimum results can be obtained at incubation temperature of 23 ± 2 °C.
    2. Calculate the mean OD for each calibrator, control and sample.
    3. Plot the mean OD readings for each of the Calibrators along the y-axis versus log of Estriol calibrator concentrations in ng/mL on the x-axis, using a 4PL or sigmoid curve fit.
    4. Determine the Estriol concentrations of the controls and samples from the calibration curve by matching their mean OD readings with the corresponding Estriol concentrations.
    5. Any sample reading higher than the highest calibrator should be appropriately diluted into a low reading serum sample and re-assayed.
    6. Any sample reading lower than the analytical sensitivity should be reported as such.
    限制
    仅限研究用
  • 注意事项
    For in vitro diagnostic use. The following precautions should be observed: a) Follow good laboratory practice. b) Use personal protective equipment. Wear lab coats and disposable gloves when handling immunoassay materials. c) Handle and dispose of all reagents and material in compliance with applicable regulations WARNING: Potential Biohazardous Material This reagent may contain some human source material (e.g. serum) or materials used in conjunction with human source materials. Handle all reagents and patient samples at a Biosafety Level 2, as recommended for any potentially infectious human material in the Centers for Disease Control/National Institutes of Health manual "Biosafety in Microbiological and Biomedical Laboratories," 5th Edition, 2007. WARNING: Potential Chemical Hazard Some reagents in this kit contain Pro-Clean 400 and sodium azide as a preservative. Pro-Clean 400 and sodium azide in concentrated amounts are irritants to skin and mucous membranes. For further information regarding hazardous substances in the kit, please refer to the MSDS.
    储存条件
    4 °C
  • 抗原 See all Estriol ELISA试剂盒
    Estriol
    Abstract
    Estriol 产品
    物质类
    Hormone
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