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CXCL10 ELISA 试剂盒

CXCL10 适用: 人, 猕猴 Colorimetric Sandwich ELISA 20-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2748281
发货至: 中国
  • 抗原 See all CXCL10 ELISA试剂盒
    CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
    适用
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    人, 猕猴
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    20-5000 pg/mL
    最低检测浓度
    20 pg/mL
    应用范围
    ELISA
    原理
    Rhesus Monkey IP-10 (CXCL10) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects Rhesus Monkey and human IP-10. Other species not determined.
    灵敏度
    20 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our top product CXCL10 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all CXCL10 ELISA试剂盒
    CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
    别名
    IP-10 (CXCL10 产品)
    别名
    CXCL10 ELISA Kit, C7 ELISA Kit, IFI10 ELISA Kit, INP10 ELISA Kit, IP-10 ELISA Kit, SCYB10 ELISA Kit, crg-2 ELISA Kit, gIP-10 ELISA Kit, mob-1 ELISA Kit, CRG-2 ELISA Kit, IP10 ELISA Kit, Ifi10 ELISA Kit, Scyb10 ELISA Kit, C-X-C motif chemokine ligand 10 ELISA Kit, C-X-C motif chemokine 10 ELISA Kit, chemokine (C-X-C motif) ligand 10 ELISA Kit, CXCL10 ELISA Kit, cxl10 ELISA Kit, Cxcl10 ELISA Kit
    背景
    C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10)
    基因ID
    574243
    UniProt
    Q8MIZ1
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