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Apoptosis Detection Kit (Annexin-V-FITC / PI)

ABIN2746064 产品详细信息, 供应商: Log in to see
抗原
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin 5
  • annexin A5-like
  • ANXA5
  • anxa5b
  • anxa5
  • LOC100220003
  • LOC100305020
  • LOC100342904
  • Anxa5
  • LOC100521982
Alternatives
绵羊 Annexin V ELISA Kit
标记
FITC
应用范围
Flow Cytometry (FACS)
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原理 This Kit is designed to rapidly analyse different stages of apoptosis and cell death in single cell suspensions.
产品特性 Annexin V binds specifically to phosphatidylserine (PS) which is located to the inner side of the cytoplasmic membrane in intact cells. But after initiation of apoptosis PS becomes rapidly translocated to the outer side of the cytoplasmic membrane where it is thought to play an important role in macrophage recognition, thus allowing the apoptotic cells to become rapidly phagocytosed. The binding of annexin V to PS is Ca2+ -dependent and therefore, a specific Ca2+ -containing reaction buffer is needed during the binding process. At low PS concentrations a binding ratio of eight annexin V molecules to one PS has been reported making annexin V- conjugates ideal for identifying membrane changes associated with early apoptotic events. In late apoptosis/necrosis cells loose their membrane integrity which allows further annexin V binding to PS located to the inner cell membrane. Therefore, MabTag's Annexin V Apoptosis Detection Kit contains a viability dye such as propidium iodide (PI). This DNAintercalating dye can only pass membranes which lost their integrity and therefore, allow the discrimination of early apoptotic cells (Annexin V+ /PI-) from late apoptotic/necrotic cells (Annexin V+ /PI+).
组件 500 μl Annexin V FITC conjugate, (with 1% BSA / 0.1% NaN3 as preservative)
500 μl propidium iodide [20 μg/ml], (w/o preservative)
10 ml Annexin V binding buffer (10fold concentrate), (w/o preservative)
试剂未包括
  • Flow cytometer or fluorescence microscope
  • Microcentrifuge
  • Microcentrifuge tubes
  • Adjustable pipettors
  • Pipette tips
  • Gloves
  • Deionized or destilled water
  • PBS and/or culture medium
别名 Annexin-V (ANXA5 ELISA Kit 摘要)
实验流程
  1. Dilute an appropriate amount of 10fold Annexin V binding buffer with deionized or
    distilled water in 1:10 ratio (mix 1 part 10x buffer with 9 parts water)
    2. Wash cells with culture medium or PBS
    3. Resuspend 104 - 106 cells in 90 μL of diluted (1 fold) Annexin V binding buffer.
    4. Add 5 μL of Annexin V conjugate and 5 μL of propidium iodide solution.
    5. Incubate for 20 minutes in the dark.
    6. Add 400 μL of Annexin V binding buffer (1fold).
    7. Centrifuge at 400 x g for 5 minutes.
    8. Resuspend cells in 200 μL Annexin V binding buffer (1fold) and analyze sample by flow cytometry or fluorescence microscopy.
限制 仅限研究用
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Upon arrival store all reagents in the dark at 2 - 8 °C. If stored properly all reagents will be stable for 12 months
有效期 12 months
厂商提供的图像
Flow Cytometry (FACS) image for Apoptosis Detection Kit (Annexin-V-FITC / PI) (ABIN2746064) 1. viable cells (double-negative)
2. early apoptotic cells (annexin V-positive...
有引用在: Honisch, Yu, Liu, Alesutan, Towhid, Tsapara, Schleicher, Handgretinger, Stournaras, Lang: "Chorein addiction in VPS13A overexpressing rhabdomyosarcoma cells." in: Oncotarget, Vol. 6, Issue 12, pp. 10309-19, 2015 (PubMed).