FITC Annexin V Apoptosis Detection Kit with PI

ABIN2669213 产品详细信息, 供应商: Log in to see
抗原
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin 5
  • annexin A5-like
  • ANXA5
  • anxa5b
  • anxa5
  • LOC100220003
  • LOC100305020
  • LOC100342904
  • Anxa5
  • LOC100521982
适用
哺乳动物
16
15
15
13
12
8
7
7
4
2
2
2
1
1
1
1
1
标记
FITC
应用范围
Multiplex Assay (MA)
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产品特性 FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.
组件 FITC Annexin V Apoptosis Detection Kit with PI
别名 Annexin V (ANXA5 ELISA Kit 摘要)
研究领域 Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
注意事项 Caution: Propidium Iodide Solution is toxigenic and mutagenic, handle with care.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store between 2°C and 8°C.
厂商提供的图像
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with PI (ABIN2669213) FITC Annexin V Apoptosis Detection Kit with PI
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with PI (ABIN2669213) FITC Annexin V Apoptosis Detection Kit with PI (Image 2)
背景 Jaishy, Zhang, Chung, Riehle, Soto, Jenkins, Abel, Cowart, Van Eyk, Abel: "Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity." in: Journal of lipid research, Vol. 56, Issue 3, pp. 546-61, 2015 (PubMed).

Wang, Zhao, Yang, Bi, Chi, Tian, Li: "High-dose alcohol induces reactive oxygen species-mediated apoptosis via PKC-β/p66Shc in mouse primary cardiomyocytes." in: Biochemical and biophysical research communications, Vol. 456, Issue 2, pp. 656-61, 2014 (PubMed).