Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) concentrations in cell culture supernates, serum, and plasma.
样品类型
Serum, Plasma, Cell Culture Cells
Analytical Method
Quantitative
灵敏度
7 pg/ml
产品特性
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
Optimal working dilution should be determined by the investigator.
样本量
100 μL
实验时间
3 h
板类型
Pre-coated
实验流程
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GM-CSF is added to the wells and binds to the combination of capture antibody- GM-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of GM-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven GM-CSF standard dilutions and GM-CSF sample concentration determined.