Myocyte Enhancer Factor 2A (MEF2A) ELISA 试剂盒

ABIN2114546 产品详细信息, 供应商: Log in to see
  • MEF2A
  • xmef2a
  • ADCAD1
  • RSRFC4
  • RSRFC9
  • mef2
  • A430079H05Rik
  • adcad1
  • rsrfc4
  • rsrfc9
  • sl2
  • mef2a
  • wu:fd19d02
  • myocyte enhancer factor 2A
  • myocyte-specific enhancer factor 2A
  • myocyte-specific enhancer factor 2a, putative
  • putative myocyte-specific enhancer factor 2a
  • myocyte enhancer factor 2a
  • myocyte enhancer factor 2A L homeolog
  • myocyte enhancer factor 2aa
  • MEF2A
  • EDI_092490
  • EDI_038250
  • Smp_161530
  • Smp_129430
  • mef2a
  • Mef2a
  • mef2a.L
  • mef2aa
Human Myocyte Enhancer Factor 2A ELISA Kit
人, 小鼠, 大鼠
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Analytical Method Quantitative
检测方法 Colorimetric
特异性 The MEF2A (Phospho-Thr312) DNA-Binding ELISA Kit detects endogenous levels of MEF2A only when phosphorylated at Thr312.
产品特性 Assay Type: DNA-Binding
别名 MEF2A (MEF2A ELISA Kit 摘要)
背景 Synonyms: MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1
Gene Symbol: MEF2A
基因ID 4205
UniProt Q02078
研究领域 Cardiovascular, Hypertrophy, Cardiogenesis, Transcription Factors, Neurogenesis
途径 Neurotrophin Signaling Pathway, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades
板类型 Pre-coated
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
限制 仅限研究用
注意事项 Avoid multiple freeze-thaw cycles
储存条件 4 °C
储存方法 Store at 4 °C for frequent use, at -20°C for infrequent use.
有效期 6 months