C-JUN ELISA 试剂盒 (Jun Proto-Oncogene)

Details for Product JUN ELISA Kit No. ABIN2065019, 供应商: Log in to see
抗原
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • Jun
  • AP-1
  • JUN
  • B230310J22Rik
  • p39
  • Junc
  • c-jun
  • AP1
  • fj36h07
  • wu:fj36h07
  • zgc:65863
  • CG2275
  • D-Jun
  • D-jun
  • D-jun/Jra
  • DJUN
  • DJun
  • Djun
  • Dmel\\CG2275
  • JRA
  • V
  • cJun
  • d-JRA
  • d-Jun
  • d-jun
  • dAP-1
  • dJRA
  • dJUN
  • dJra
  • dJun
  • djun
  • dm-Jun
  • jra
  • jun
  • l(2)46Ef
  • l(2)IA109
  • l(2R)IA109
  • jun oncogene
  • basket
  • C-JUN protein
  • c-Jun protein
  • c-jun transcription factor
  • cyclin-dependent kinase 5, regulatory subunit 2 (p39)
  • Jun oncogene
  • jun proto-oncogene
  • Jun-related antigen
  • jun
  • bsk
  • C-JUN
  • Cdk5r2
  • Jun
  • JUN
  • Jra
Alternatives
Mouse (Murine) C-JUN ELISA Kit
抗原表位
pThr239
适用
人, 小鼠, 大鼠
其他选择
Kits with alternative reactivity to:
54
49
39
5
4
4
4
3
3
3
2
2
实验类型
DNA-Binding ELISA
应用范围
ELISA
选项
Supplier
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Supplier Product No.
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Analytical Method Quantitative
检测方法 Colorimetric
特异性 The c-Jun (Phospho-Thr239) DNA-Binding ELISA Kit detects endogenous levels of c-Jun only when phosphorylated at Thr239.
产品特性 Assay Type: DNA-Binding
别名 C-Jun (JUN ELISA Kit 摘要)
背景 Synonyms: Activator protein 1, AH119, AP-1, AP1, c-Jun, Jun A, p39, Proto-oncogene c-jun, RJG-9, V-jun avian sarcoma virus 17 oncogene homolog
Gene Symbol: JUN
基因ID 3725
UniProt P05412
研究领域 Immunology, Innate Immunity, Transcription Factors
途径 MAPK Pathway, RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor
板类型 Pre-coated
实验流程
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
限制 仅限研究用
注意事项 Avoid multiple freeze-thaw cycles
储存条件 4 °C
储存方法 Store at 4 °C for frequent use, at -20°C for infrequent use.
有效期 6 months
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