Mitochondrial Permeability Transition Pore Assay Kit

ABIN2015837 产品详细信息, 供应商: Log in to see
适用
哺乳动物
应用范围
Activity Assay (AcA), Detection (D), Flow Cytometry (FACS)
选项
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样品类型 Biological Fluids, Adherent Cell Culture
检测方法 Fluorometric
产品特性 Simple procedure
Fast, convenient and sensitive
The assay provides a direct method of measuring mitochondrial permeability transition pore activation as an early marker of apoptosis
组件 MPTP Staining Dye
CoCl2
Ionomycin
MPTP Wash Buffer
背景 Mitochondria are the power centrals of the cell and play an essential role in energy production. Damage to mitochondria activates signaling pathways that induce apoptosis. The mitochondrial permeability transition pore (MPT pore or MPTP) is a nonspecific channel formed by components of the inner and outer mitochondrial membranes, and appears to be involved in the release of mitochondrial components during cell death. In healthy cells, MPTP's flicker between open and closed states but during cell death MPTP's dramatically alter the permeability of the mitochondria. Cytochrome c release and loss of mitochondrial membrane potential are subsequent to continuous pore activation.
应用备注 Measure mitochondrial permeability transition pore activity in cells Assessment of physiological mediators and antibodies that affect cell viability/cytotoxicity
说明

Further details regarding sample type: Cells (adherent and suspension)

限制 仅限研究用
储存条件 -20 °C
有效期 12 months
厂商提供的图像
Flow Cytometry (FACS) image for Mitochondrial Permeability Transition Pore Assay Kit (ABIN2015837) Mitochondrial Permeability Transition Pore Assay Kit
有引用在: Li, Zhao, Zhou, Zhang, Qin: "Exendin-4 promotes endothelial barrier enhancement via PKA- and Epac1-dependent Rac1 activation." in: American journal of physiology. Cell physiology, Vol. 308, Issue 2, pp. C164-75, 2015 (PubMed).