MK2-Trap

ABIN1889472 产品详细信息, 供应商: Log in to see
抗原
  • mapkapk2
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
  • MGC78852
  • MAPKAPK2
  • AA960234
  • MAPKAP-K2
  • MK-2
  • MK2
  • Rps6kc1
  • mitogen-activated protein kinase-activated protein kinase 2a
  • mitogen-activated protein kinase-activated protein kinase 2
  • MAP kinase-activated protein kinase 2
  • mapkapk2a
  • mapkapk2
  • MAPKAPK2
  • Mapkapk2
Alternatives
Human MAPKAP Kinase 2 ELISA Kit
适用
Hamster, 人, 小鼠
11
11
9
2
宿主
Camelidae
抗体类型
Recombinant Antibody
应用范围
Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif)
选项
Supplier
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原理 The MK2-Trap is a high quality MK2-binding protein for biochemical analysis of MK2 and interacting partners.
样品类型 Cell Extracts
特异性 Posttranslational Modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.
产品特性 MK2-Trap is excellent for fast and efficient one-step isolation of MK2 and its interacting factors from cellular extract. Isolated MK2 protein may be used further for immunoblot analysis, mass spectrometry, and kinase assays.
MK2-Trap utilizes small recombinant alpaca antibody fragments covalently coupled to the surface of agarose beads.
纯化方法 Purifed protein
组件 MK2-Trap uncoupled
试剂未包括 Lysis buffer (CoIP), 10x RIPA buffer, Dilution buffer, Wash buffer, Elution buffer
别名 MK2 (MAPKAPK2 ELISA Kit 摘要)
背景 Mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2 / MK2) belongs to the family of serine/threonine kinases. In response to cellular stress it is phosphorylated and activated by MAP kinase p38.
说明

Use this MK2-Trap to couple it to your own immobilizing matrix.

实验时间 1.5 h
实验流程
  • Robust and versatile tool for biochemical analyses of MK2 proteins
  • Short incubation times (5 - 30 min)
  • Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
  • Low unspecific binding
  • No contaminating heavy and light chains of conventional antibodies
  • Applicable in Chromatin Immunoprecipitation (ChIP)
样品收集 Harvest cells:
For one immunoprecipitation reaction the use of 10^6 - 10^7 mammalian cells (approx. one 10 cm dish) is recommended. To harvest adherent cells, aspirate growth medium, add 1 ml ice-cold PBS to cells and scrape cells from dish. Transfer cells to a pre-cooled tube, spin at 500 g for 3 min at +4°C and discard supernatant. Wash cell pellet twice with ice-cold PBS, gently resuspending the cells.

Lyse cells
  • 1. Resuspend cell pellet in 200 µL ice-cold lysis buffer by pipetting or using a syringe.
    note: Supplement lysis buffer with protease inhibitors and 1 mM PMSF (not included). optional for nuclear/chromatin proteins: Use RIPA buffer supplemented with 1 mg/mL DNase, 2.5 mM MgCl2, protease inhibitors and 1 mM PMSF (not included).
  • 2. Place the tube on ice for 30 min with extensively pipetting every 10 min.
  • 3. Centrifuge cell lysate at 20.000x g for 10 min at +4°C. Transfer lysate to a pre-cooled tube. Add 300 µl dilution buffer to lysate. Discard pellet.
    note: At this point cell lysate may be put at -80°C for long-term storage. optional: Add 1 mM PMSF and protease inhibitors (not included) to dilution buffer

We recommend that during incubation with the beads the final concentration of detergents does not exceed 0.2% to avoid unspecific binding to the matrix. If required, use more dilution buffer to dilute the supernatant accordingly.
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 1 x PBS, 0.01 % Sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Do not freeze.
储存条件 4 °C
有效期 12 months
厂商提供的图像
Immunoprecipitation (IP) image for MK2-Trap (ABIN1889472) Pulldown of MK2 using the MK2-Trap: Immunoprecipitations (IP) of MK2 from a protein e...
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