(9 references)-
- 抗原 See all Caspase 3 (CASP3) 试剂盒
- Caspase 3 (CASP3)
- 检测方法
- Fluorometric
- 应用范围
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- 品牌
- SensoLyte®
- 产品特性
- The SensoLyte® Homogeneous Rh110 Capase-3/7 Assay Kit uses (Ac-DEVD)2-Rh110 as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, (Ac-DEVD)2-Rh110 generates the Rh110 fluorophore which has bright green fluorescence and can be detected at Ex/Em=496 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from cell components.A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
- Top Product
- Discover our top product CASP3 ELISA Kit
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Caspase 3 (CASP3) ELISA Kit
CASP3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
SensoLyte® Homogeneous AMC Caspase-3/7 Assay KitFRET Fluorometric
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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FRET-based Assay Kit
- 限制
- 仅限研究用
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- 注意事项
- Protect Components A and B from light and from moisture.
- 储存条件
- -20 °C
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: "Curcumin blocks brain tumor formation." in: Brain research, Vol. 1266, pp. 130-8, (2014) (PubMed).
: "Selective serotonin reuptake inhibitors (SSRIs) inhibit insulin secretion and action in pancreatic ? cells." in: The Journal of biological chemistry, Vol. 288, Issue 8, pp. 5682-93, (2013) (PubMed).
: "Distinct roles of mitochondria- and ER-localized Bcl-xL in apoptosis resistance and Ca2+ homeostasis." in: Molecular biology of the cell, Vol. 23, Issue 13, pp. 2605-18, (2012) (PubMed).
: "HIF-1 mediates pathogenic inflammatory responses to intestinal ischemia-reperfusion injury." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 4, pp. G833-43, (2010) (PubMed).
: "Nitrated {alpha}-synuclein-induced alterations in microglial immunity are regulated by CD4+ T cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 7, pp. 4137-49, (2009) (PubMed).
: "Suppression of atherogenesis by overexpression of glutathione peroxidase-4 in apolipoprotein E-deficient mice." in: Free radical biology & medicine, Vol. 44, Issue 3, pp. 343-52, (2008) (PubMed).
: "The effect of proteasome inhibition on p53 degradation and proliferation in tonsil epithelial cells." in: Archives of otolaryngology--head & neck surgery, Vol. 134, Issue 2, pp. 157-63, (2008) (PubMed).
: "Measuring the secretion of heat shock proteins from cells." in: Methods (San Diego, Calif.), Vol. 43, Issue 3, pp. 176-83, (2007) (PubMed).
: "Human papillomavirus type 16 E6 activates NF-kappaB, induces cIAP-2 expression, and protects against apoptosis in a PDZ binding motif-dependent manner." in: Journal of virology, Vol. 80, Issue 11, pp. 5301-7, (2006) (PubMed).
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: "Curcumin blocks brain tumor formation." in: Brain research, Vol. 1266, pp. 130-8, (2014) (PubMed).
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- 抗原
- Caspase 3 (CASP3)
- 别名
- Caspase-3 (CASP3 产品)
- 别名
- CPP32 Kit, CPP32B Kit, SCA-1 Kit, A830040C14Rik Kit, AC-3 Kit, Apopain Kit, CC3 Kit, Caspase-3 Kit, Lice Kit, Yama Kit, mldy Kit, xcpp32 Kit, casp3 Kit, zgc:100890 Kit, CASP-3 Kit, caspase-3 Kit, caspase 3 Kit, caspase 3 S homeolog Kit, caspase 3, apoptosis-related cysteine peptidase a Kit, caspase 3, apoptosis-related cysteine peptidase Kit, CASP3 Kit, Casp3 Kit, casp3.S Kit, casp3a Kit
- 背景
- Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
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