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TNFRSF4 ELISA 试剂盒

TNFRSF4 适用: 小鼠 AA 20-211 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN1672893
发货至: 中国
  • 抗原 See all TNFRSF4 ELISA试剂盒
    TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
    抗原表位
    AA 20-211
    适用
    • 4
    • 4
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2-2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF4/OX40
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: V20-P211
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: V20-P211
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    板类型
    Pre-coated
    实验流程
    mouse TNFRSF4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF4 has been precoated onto 96-well plates. Standards(NSO, V20-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF4 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFRSF4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF4 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 224, Standard deviation: 7.62, CV(%): 3.4
    • Sample 2: n=16, Mean(pg/ml): 732, Standard deviation: 32.94, CV(%): 4.5
    • Sample 3: n=16, Mean(pg/ml): 1463, Standard deviation: 83.4, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 364, Standard deviation: 17.5, CV(%): 4.8
    • Sample 2: n=24, Mean(pg/ml): 885, Standard deviation: 49.56, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 1376, Standard deviation: 95, CV(%): 6.9
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all TNFRSF4 ELISA试剂盒
    TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
    别名
    TNFRSF4 (TNFRSF4 产品)
    别名
    TNFRSF4 ELISA Kit, ACT35 ELISA Kit, CD134 ELISA Kit, Ly-70 ELISA Kit, Ox40 ELISA Kit, TXGP1L ELISA Kit, Txgp1 ELISA Kit, OX40 ELISA Kit, TNF receptor superfamily member 4 ELISA Kit, tumor necrosis factor receptor superfamily, member 4 ELISA Kit, TNFRSF4 ELISA Kit, Tnfrsf4 ELISA Kit
    背景

    Protein Function: Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity). .

    Background: Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.

    Synonyms: Tumor necrosis factor receptor superfamily member 4,OX40 antigen,OX40L receptor,CD134,Tnfrsf4,Ox40, Txgp1,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 4

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    基因ID
    22163
    UniProt
    P47741
    途径
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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