2B4 ELISA 试剂盒
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- 抗原 See all 2B4 (CD244) ELISA试剂盒
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 抗原表位
- AA 22-221
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD244/2B4
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: C22-R221 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: C22-R221 - Featured
- Discover our best selling CD244 ELISA Kit
- Top Product
- Discover our top product CD244 ELISA Kit
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Natural Killer Cell Receptor 2B4 (CD244) ELISA Kit
CD244 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Natural Killer Cell Receptor 2B4 (CD244) ELISA KitCD244 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Natural Killer Cell Receptor 2B4 (CD244) ELISA KitCD244 适用: 人 Colorimetric Sandwich ELISA 0.625-40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. .
- 板类型
- Pre-coated
- 实验流程
- human CD244 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD244 has been precoated onto 96-well plates. Standards(NSO, C22-R221) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD244 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD244 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD244 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD244 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 0.45, Standard deviation: 0.022, CV(%): 4.9
- Sample 2: n=16, Mean(ng/ml): 1.2, Standard deviation: 0.076, CV(%): 6.3
- Sample 3: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.186, CV(%): 5.8,
- Sample 1: n=24, Mean(ng/ml): 0.43, Standard deviation: 0.027, CV(%): 6.2
- Sample 2: n=24, Mean(ng/ml): 1.3, Standard deviation: 0.098, CV(%): 7.5
- Sample 3: n=24, Mean(ng/ml): 2.9, Standard deviation: 0.194, CV(%): 6.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all 2B4 (CD244) ELISA试剂盒
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 别名
- CD244 (CD244 产品)
- 别名
- CD244 ELISA Kit, 2B4 ELISA Kit, NAIL ELISA Kit, NKR2B4 ELISA Kit, Nmrk ELISA Kit, SLAMF4 ELISA Kit, C9.1 ELISA Kit, F730046O15Rik ELISA Kit, Ly90 ELISA Kit, CD244 molecule ELISA Kit, CD244 natural killer cell receptor 2B4 ELISA Kit, CD244 ELISA Kit, Cd244 ELISA Kit
- 背景
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Protein Function: Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. .
Background: CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
Synonyms: Natural killer cell receptor 2B4,NK cell activation-inducing ligand,NAIL,NK cell type I receptor protein 2B4,NKR2B4,h2B4,SLAM family member 4,SLAMF4,Signaling lymphocytic activation molecule 4,CD244,CD244,2B4,
Full Gene Name: Natural killer cell receptor 2B4
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 51744
- UniProt
- Q9BZW8
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