CCL1 ELISA 试剂盒
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- 抗原 See all CCL1 ELISA试剂盒
- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
- 抗原表位
- AA 24-96
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: K24-K96 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <4pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: K24-K96 - Featured
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- Discover our top product CCL1 ELISA Kit
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Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
CCL1 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 小鼠 AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
- 板类型
- Pre-coated
- 实验流程
- human CCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL1 has been precoated onto 96-well plates. Standards(NSO, K24-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 46, Standard deviation: 2.1, CV(%): 4.6
- Sample 2: n=16, Mean(pg/ml): 125, Standard deviation: 6.4, CV(%): 5.1
- Sample 3: n=16, Mean(pg/ml): 738, Standard deviation: 53.1, CV(%): 7.2,
- Sample 1: n=24, Mean(pg/ml): 48, Standard deviation: 3.0, CV(%): 6.3
- Sample 2: n=24, Mean(pg/ml): 127, Standard deviation: 8.5, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 741, Standard deviation: 54.8, CV(%): 7.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CCL1 ELISA试剂盒
- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
- 别名
- CCL1 (CCL1 产品)
- 别名
- I-309 ELISA Kit, P500 ELISA Kit, SCYA1 ELISA Kit, SISe ELISA Kit, TCA3 ELISA Kit, BF534335 ELISA Kit, Scya1 ELISA Kit, Tca-3 ELISA Kit, CCLi6 ELISA Kit, chemokine ELISA Kit, C-C motif chemokine ligand 1 ELISA Kit, chemokine (C-C motif) ligand 1 ELISA Kit, CCL1 ELISA Kit, Ccl1 ELISA Kit
- 背景
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Protein Function: Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. .
Background: CCL1, Chemokine(C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
Synonyms: C-C motif chemokine 1,Small-inducible cytokine A1,T lymphocyte-secreted protein I-309,CCL1,SCYA1,
Full Gene Name: C-C motif chemokine 1
Cellular Localisation: Secreted. - 基因ID
- 6346
- UniProt
- P22362
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