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CTH ELISA 试剂盒

CTH 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
产品编号 ABIN1569201
发货至: 中国
  • 抗原 See all CTH ELISA试剂盒
    CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    原理
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CSE in rat tissue homogenates and other biological fluids.
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of this index.
    交叉反应 (详细)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    灵敏度
    0.053 ng/mL
    组件
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 样本量
    100 μL
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    实验流程

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    实验精密度
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    注意事项
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    储存条件
    4 °C,-20 °C
    储存方法
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    有效期
    12 months
  • 抗原 See all CTH ELISA试剂盒
    CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))
    别名
    Cystathionine Gamma Lyase (CSE) (CTH 产品)
    别名
    DYT17 ELISA Kit, DYT18 ELISA Kit, DYT9 ELISA Kit, EIG12 ELISA Kit, GLUT ELISA Kit, GLUT1 ELISA Kit, GLUT1DS ELISA Kit, HTLVR ELISA Kit, PED ELISA Kit, wu:fb48e03 ELISA Kit, wu:fb58d08 ELISA Kit, zgc:66001 ELISA Kit, zgc:85785 ELISA Kit, CTH ELISA Kit, 0610010I13Rik ELISA Kit, AI314617 ELISA Kit, BC019483 ELISA Kit, CSE ELISA Kit, Cys3 ELISA Kit, cysA ELISA Kit, SCQ11.03c ELISA Kit, DDBDRAFT_0190313 ELISA Kit, DDBDRAFT_0191318 ELISA Kit, DDB_0190313 ELISA Kit, DDB_0191318 ELISA Kit, H ELISA Kit, MGC75946 ELISA Kit, cystathionine gamma-lyase ELISA Kit, solute carrier family 2 member 1 ELISA Kit, cystathionase (cystathionine gamma-lyase) ELISA Kit, cystathionine gamma-lyase L homeolog ELISA Kit, CTH ELISA Kit, SLC2A1 ELISA Kit, cth ELISA Kit, Cth ELISA Kit, SCO3920 ELISA Kit, mlr1566 ELISA Kit, TA_RS00415 ELISA Kit, MAP_RS15640 ELISA Kit, SPO3344 ELISA Kit, CNN01730 ELISA Kit, Mmcs_1928 ELISA Kit, RHA1_RS31820 ELISA Kit, HNE_1212 ELISA Kit, MAV_3867 ELISA Kit, MSMEG_2394 ELISA Kit, Mvan_2142 ELISA Kit, SACE_6136 ELISA Kit, Mflv_4220 ELISA Kit, cysA ELISA Kit, cth.L ELISA Kit
    背景
    Alternative name: CTH, Cystathionase, Cysteine-protein sulfhydrase, Gamma-cystathionase
    基因ID
    24962
    UniProt
    P18757
    途径
    ER-Nucleus Signaling, Warburg Effect
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