Annexin V-FITC Apoptosis Detection Kit

ABIN1379891 产品详细信息, 供应商: Log in to see
抗原
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin 5
  • annexin A5-like
  • ANXA5
  • anxa5b
  • anxa5
  • LOC100220003
  • LOC100305020
  • LOC100342904
  • Anxa5
  • LOC100521982
Alternatives
Human Annexin V ELISA Kit
标记
FITC
应用范围
Fluorescence Microscopy (FM), Flow Cytometry (FACS)
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原理 The assay combines annexin V staining of PS membrane events with the staining of cell nucleus with PI or AAD-7 in living cells to distinguish from dead cells.
检测方法 Fluorometric
产品特性 Annexin A5 is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (annexin V binding to PS is Ca2+ dependent). Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy
别名 Annexin V (ANXA5 ELISA Kit 摘要)
背景 Annexin A5 (or Annexin V) is a cellular protein in the annexin group. Annexin A5 is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death.
研究领域 Apoptosis
板类型 Strips (12 x 8)
实验流程

Staining Protocol:
1.Dilute the Binding buffer (10×) to work solution (1×) with distilled water.
2.Harvest cell, then wash once with cold PBS, remove the PBS from the cell pellet.
3.Wash again with cold 1× Binding buffer, remove the Binding buffer from the cell pellet.
4.Resuspend cells in cold 1× Binding buffer to a concentration of 1×10 6 cells/mL.
5.Add 100µL of cells (1×10 5 ) to each appropriate tube.
6.Add 5µL of Annexin V-FITC to appropriate tubes.
7.Gently vortex each tube and incubate for 10 minutes at room temperature in dark.
8.Add 5µL of PI solution and incubate for 5 min at room temperature in dark.
9.Wash cells once in PBS and resuspend in PBS.
10.Analyze by flow cytometry within 4 hours.

限制 仅限研究用
储存液 Sodium azide, Thimerosal (Merthiolate)
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