IgG ELISA 试剂盒
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- 抗原 See all IgG ELISA试剂盒
- IgG
- 抗原表位
- Heavy & Light Chain
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 20-1900 ng/mL
- 最低检测浓度
- 20 ng/mL
- 应用范围
- ELISA
- 原理
- Rapid quantification of mouse IgG in cell culture supernatant, follow-up of production process
- 品牌
- FastELISA®
- 样品类型
- Cell Culture Supernatant, Ascitic Fluid
- Analytical Method
- Quantitative
- 特异性
- The method enables the detection of all IgG (IgG3 quantification requires a specific standard curve).
- 交叉反应 （详细）
- < 1% for Human IgG, < 1.5% for Cow IgG, < 2% for Goat IgG, < 5 % for Swine IgG and < 15 % for Guinea Pig and Rat IgG. The cross reaction with human serum and fetal calf serum is typically below 0.2%.
- 灵敏度
- 6 ng/mL
- 产品特性
- The mouse immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
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- Discover our best selling IgG ELISA Kit
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- Discover our top product IgG ELISA Kit
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IgG ELISA Kit
适用: 兔 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 人 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Rabbit TrueBlot® Set (with IP beads)WB, IP 适用: 兔
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- 说明
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One Step ELISA 30 min total time
- 实验时间
- < 1 h
- 板类型
- Pre-coated
- 实验流程
- A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- 试剂准备
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dilution
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all IgG ELISA试剂盒
- IgG
- 别名
- Immunoglobulin G (IgG 产品)
- 物质类
- Antibody
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