Pulldown of GST using the GST-Trap Immunoprecipitations (IP) of GST from protein extracts of GST-expressing E.coli cells. Input (I), non-bound (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie staining and Western Blotting. Unlike conventional antibodies, the GST-Trap is free from heavy and/or light chain contaminants and therefore yields cleaner results.
Protein lysate pf E.coli cells expressing GST was spearated by SDS-Page and analyzed by Western blot using GST antibody 6G9 (1:500). GST antibody 6G9 is highly specific for GST and shows no cross reactivity to other E.coli proteins.