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Human IgG isotype control (Biotin)

ABIN964324 产品详细信息, 供应商: Log in to see
亚型
宿主
Human
217
207
204
168
160
127
112
93
88
88
88
73
56
50
43
42
27
25
24
22
21
11
8
3
3
2
1
抗体类型
Native
标记
Biotin
205
169
167
166
154
108
58
58
48
44
19
13
12
12
11
9
9
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
应用范围
Isotype Control (IsoC), ELISA, Western Blotting (WB)
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亚型 IgG
片段 Fc fragment
产品特性 Concentration Definition: by UV absorbance at 280 nm
背景 Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site. This Human IgG whole molecule is conjugated to biotin (Vitamin H), a small biomolecule that has a large affinity for avidin and streptavidin.

Synonyms: Human Immunoglobulin G F(c) fragment, IgG Fc, Vitamin H, Coenzyme R
研究领域 Immunology, Secondary Antibodies
应用备注 Human IgG F(c) fragment Biotin conjugated can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
限制 仅限研究用
状态 Lyophilized
溶解方式 Restore with deionized water (or equivalent)
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Aliquot to Avoid repeated freezing and thawing.
储存条件 4 °C