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Human IgG isotype control (PE)

FACS, WB, IHC IgG PE
产品编号 ABIN5633236
发货至: 中国
  • 抗原 See all IgG products
    IgG
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    Human
    标记
    PE
    应用范围
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)
    交叉反应 (详细)
    Human IgG whole molecule Phycoerythrin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Human IgG and anti-Human Serum.
    纯化方法
    Human IgG whole molecule Phycoerythrin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    亚型
    IgG
    Labeling Ratio
    1-2
  • 应用备注

    Flow Cytometry Dilution: 1:500-1:2500

    Immunohistochemistry Dilution: 1:500-1:2500

    Application Note: Human IgG whole molecule Phycoerythrin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

    Western Blot Dilution: 1:1,000-1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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