电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

小鼠 IgG2b isotype control (APC)

FACS, ELISA, FLISA, FM Monoclonal IgG2b APC
产品编号 ABIN5608811
发货至: 中国
  • 抗原 See all IgG2b products
    IgG2b
    适用
    小鼠
    宿主
    • 128
    • 51
    • 2
    小鼠
    克隆类型
    • 120
    • 2
    • 1
    单克隆
    标记
    APC
    应用范围
    Flow Cytometry (FACS), ELISA, FLISA, Fluorescence Microscopy (FM)
    纯化方法
    Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3 , IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition has been confirmed.
    亚型
    IgG2b
    Labeling Ratio
    1-2
  • 应用备注

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: Mouse IgG2b kappa isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

    FLISA Dilution: User Optimized

    ELISA Dilution: 1:2000-1:20,000

    IF Microscopy Dilution: User Optimized

    说明

    Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 3 and μ - IgM, light chain κ and λ.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Stabilizer: None

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
    有效期
    12 months
  • 抗原
    IgG2b
    Abstract
    IgG2b 产品
    别名
    IgG2b Isotype Control, Igh-3 Isotype Control, gamma2b Isotype Control, immunoglobulin heavy constant gamma 2B Isotype Control, Ighg2b Isotype Control
    物质类
    Antibody
You are here:
客服