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大鼠 IgG2a isotype control (FITC)

FACS, ELISA, FLISA, FM Monoclonal IgG2a FITC
产品编号 ABIN5608809
发货至: 中国
  • 抗原 See all IgG2a products
    IgG2a
    适用
    大鼠
    宿主
    • 171
    • 65
    • 3
    • 1
    大鼠
    克隆类型
    • 171
    • 2
    • 1
    单克隆
    标记
    FITC
    应用范围
    Flow Cytometry (FACS), ELISA, FLISA, Fluorescence Microscopy (FM)
    纯化方法
    RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1, IgG2b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
    亚型
    IgG2a
    Labeling Ratio
    2-8
  • 应用备注

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: RAT IgG2a isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

    FLISA Dilution: User Optimized

    ELISA Dilution: 1:2000-1:20,000

    IF Microscopy Dilution: User Optimized

    说明

    IgG2a isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 2c and μ - IgM, light chain κ and λ.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Stabilizer: None

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
    有效期
    12 months
  • 抗原
    IgG2a
    Abstract
    IgG2a 产品
    别名
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    物质类
    Antibody
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