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Llama IgG1 isotype control (FITC)

FACS, WB, FM, FLISA IgG1 FITC
产品编号 ABIN5608792
发货至: 中国
  • 抗原 See all IgG1 products
    IgG1
    适用
    Llama
    宿主
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    Llama
    标记
    FITC
    应用范围
    Flow Cytometry (FACS), Western Blotting (WB), Fluorescence Microscopy (FM), FLISA
    纯化方法
    Llama IgG1 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain). No bands corresponding to llama IgG2 or IgG3 are observed.
    亚型
    IgG1
    Labeling Ratio
    3.2
  • 应用备注

    Flow Cytometry Dilution: User Optimized

    Application Note: Llama IgG1 FITC Conjugated isotype control can be utilized as a control or standard reagent in Flow Cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting experiments where determination of sample isotype is important.

    Western Blot Dilution: User Optimized

    FLISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    说明

    Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35 % - 70 % of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses. All gamma chain camelid antibodies exhibiting the more conventional assembly of two light and two heavy chains with molecular weight ~150 kDa, belong to the IgG1 subclass.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    IgG1
    Abstract
    IgG1 产品
    别名
    IgG1 Isotype Control, Igh-4 Isotype Control, VH7183 Isotype Control, immunoglobulin heavy constant gamma 1 (G1m marker) Isotype Control, Ighg1 Isotype Control
    物质类
    Antibody
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