仓鼠 IgG isotype control (Biotin)

ABIN457793 产品详细信息, 供应商: Log in to see
亚型
宿主
仓鼠
219
210
208
172
162
129
114
94
93
92
91
76
59
52
44
42
27
25
24
23
22
11
8
6
3
3
1
克隆类型
多克隆
标记
Biotin
206
169
168
166
157
108
58
58
48
45
20
13
12
12
11
9
9
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunocytochemistry (ICC), Isotype Control (IsoC), Immunohistochemistry (IHC), ELISA
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Supplier
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免疫原 Purified normal IgG isolated from pooled Syrian hamster serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
亚型 IgG
片段 H&L
特异性 The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule.
产品特性 Biotin-conjugated IgG fraction of polyclonal hamster antiserum to hamster IgG, heavy and light chains
Biotin/IgG protein molar ratio (B/P) is approximately 4.5.
纯化方法 No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
研究领域 Immunology, Secondary Antibodies
应用备注 In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of hamster origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:6,000.
限制 仅限研究用
状态 Lyophilized
溶解方式 It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
浓度 10 mg/mL
缓冲液 Biotin-coupled purified hyperimmune hamster IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
储存液 Without preservative
注意事项 Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day . If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
储存条件 4 °C/-20 °C
储存方法 The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.