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小鼠 IgG1 同型对照

Name: 小鼠 IgG1 同型对照
Brand: antibodies-online
SKU: ABIN457406
Availability: OutOfStock

FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG1 Isotype control (MOPC-21) purified antibody (low endotoxin, concentration in sample 9 μg/mL).

FACS

1 image

(9 references)

产品编号 ABIN457406

发货至: 中国

Datasheet as PDF

抗原 See all IgG1 products

IgG1
宿主
208

36

27

9

4

4

2

1

1
小鼠
克隆类型
176

11

6
单克隆

应用范围

Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

特异性

This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.

无交叉反应

人, 大鼠

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

内毒素水平

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
克隆位点
43

21

17

12

7

6

6

5

5

4

4

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
MOPC-21

亚型

IgG1 kappa
Mouse IgG1 isotype control (Biotin)
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin

Mouse IgG1 isotype control (APC)
FACS, IsoC, IF IgG1 APC

Mouse IgG1 isotype control (FITC)
FACS, ELISA Monoclonal 15H6 IgG1 FITC

Mouse IgG1 isotype control (PE)
FACS, ELISA Monoclonal 15H6 IgG1 PE

Mouse IgG1 isotype control (SPRD)
FACS, ELISA Monoclonal 15H6 IgG1 SPRD

Mouse IgG1 isotype control (APC)
FACS, ELISA Monoclonal 15H6 IgG1 APC

Mouse IgG1 isotype control
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control (Biotin)
FACS, ELISA Monoclonal 15H6 IgG1 Biotin

Mouse IgG1 isotype control (PE-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7

Mouse IgG1 isotype control (APC-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7

应用备注

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存液

Azide free

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Piao, Na, Seo, Hong, Sohn, Kwon, Lee, Kim: "Effects of general anaesthesia with an inhalational anaesthetic agent on the expression of exosomes in rats." in: International journal of medical sciences, Vol. 19, Issue 9, pp. 1399-1407, (2022) (PubMed).

Ni, Zhou, Song, Chen, Wang, Du, Zhang: "ELAVL1a is an immunocompetent protein that protects zebrafish embryos from bacterial infection." in: Communications biology, Vol. 4, Issue 1, pp. 251, (2021) (PubMed).

Klaile, Müller, Schäfer, Clauder, Feer, Heyl, Stock, Klassert, Zipfel, Singer, Slevogt: "Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells." in: mBio, Vol. 8, Issue 2, (2017) (PubMed).

Marchiani, Tamburrino, Giuliano, Nosi, Sarli, Gandini, Piomboni, Belmonte, Forti, Baldi, Muratori: "Sumo1-ylation of human spermatozoa and its relationship with semen quality." in: International journal of andrology, Vol. 34, Issue 6 Pt 1, pp. 581-93, (2011) (PubMed).

Rebetz, Tian, Persson, Widegren, Salford, Englund, Gisselsson, Fan: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).

Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, (2007) (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).

Bryceson, March, Barber, Ljunggren, Long: "Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells." in: The Journal of experimental medicine, Vol. 202, Issue 7, pp. 1001-12, (2005) (PubMed).
抗原

IgG1

Abstract

IgG1 产品

别名

IgG1 Isotype Control, Igh-4 Isotype Control, VH7183 Isotype Control, immunoglobulin heavy constant gamma 1 (G1m marker) Isotype Control, Ighg1 Isotype Control

物质类

Antibody