小鼠 IgG1 同型对照

ABIN457406 产品详细信息, 供应商: Log in to see
亚型
  • IgG1
  • Igh-4
  • VH7183
  • immunoglobulin heavy constant gamma 1 (G1m marker)
  • Ighg1
宿主
小鼠
267
86
22
4
3
3
2
2
1
1
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Negative Control (NC), Western Blotting (WB)
选项
Supplier
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Supplier Product No.
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克隆位点 MOPC-21
亚型 IgG1
特异性 This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
无交叉反应 人, 大鼠
纯化方法 Purified from cell culture supernatant by protein-G affinity chromatography.
纯度 > 95 % (by SDS-PAGE)
过滤 0.2 μm filtered
内毒素水平 Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
背景 The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ,g/mL) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 Molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
研究领域 Secondary Antibodies
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
浓度 1 mg/mL
缓冲液 Azide free phosphate buffered saline (PBS), approx. pH 7.4.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
有引用在: Marchiani, Tamburrino, Giuliano, Nosi, Sarli, Gandini, Piomboni, Belmonte, Forti, Baldi, Muratori: "Sumo1-ylation of human spermatozoa and its relationship with semen quality." in: International journal of andrology, Vol. 34, Issue 6 Pt 1, pp. 581-93, 2011 (PubMed).

Rebetz, Tian, Persson, Widegren, Salford, Englund, Gisselsson, Fan: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1936, 2008 (PubMed).

Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, 2008 (PubMed).

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, 2007 (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, 2007 (PubMed).

Bryceson, March, Barber, Ljunggren, Long: "Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells." in: The Journal of experimental medicine, Vol. 202, Issue 7, pp. 1001-12, 2005 (PubMed).