Human IgG isotype control (FITC)

ABIN398849 产品详细信息, 供应商: Log in to see
亚型
宿主
Human
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标记
FITC
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应用范围
Blocking Reagent (BR), Isotype Control (IsoC)
选项
Supplier
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Supplier Product No.
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亚型 IgG
片段 Whole molecule
产品特性 Purified Human IgG (Whole Molecule) Control [FITC] is highly purified from normal human serum through Protein G chromatography. It is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunoblotting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction.
Absorption Wavelength: 492 nm,
Emission Wavelength: 518 nm,
Fluorochrome/Protein Ratio: 4.1 moles FITC per mole of IgG
纯化方法 Protein G chromatography
研究领域 Immunology, Secondary Antibodies
应用备注 Each Investigator should determine their own optimal working dilution for specific applications.
说明

Excitation/Emission wavelength: 494 nm/514 nm

限制 仅限研究用
状态 Lyophilized
浓度 0.5 mg/mL
缓冲液 PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing.
Product is photosensitive and should be protected from light.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
有引用在: Tomita, Fuchimoto, Mori, Kato, Uemura, Handa, Tazawa, Ohdan, Okamoto, Kuroda: "Production of anti-ABO blood group antibodies after minor ABO-incompatible bone marrow transplantation in NOD/SCID/gamma(c)(null) mice." in: Clinical transplantation, Vol. 27, Issue 6, pp. E702-8, 2013 (PubMed).

Lim, Kim, Aref, Kamm, Raghunath: "Complementary effects of ciclopirox olamine, a prolyl hydroxylase inhibitor and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting." in: Integrative biology : quantitative biosciences from nano to macro, Vol. 5, Issue 12, pp. 1474-84, 2013 (PubMed).