HEK-293 Cells IgG isotype control (AVI tag,Biotin)
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- 抗原 See all IgG products
- IgG
- 片段
- Fc fragment
- 生物活性
- Active
- 适用
- 小鼠
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宿主
- HEK-293 Cells
- 标记
- AVI tag,Biotin
- 应用范围
- Isotype Control (IsoC)
- 品牌
- MABSol®,PrecisionAvi
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- This protein carries an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 36 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 亚型
- IgG
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Rabbit IgG isotype control
IsoC, ELISA, WB IgG Native unconjugated
Chicken IgG isotype controlELISA, WB, IHC IgG unconjugated
Mouse IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)ELISA, FACS Polyclonal IgG PE
Goat IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlELISA, WB, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- 缓冲液
- 50 mM Tris, 100 mM Glycine, pH 7.5
- 储存条件
- -20 °C
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
- 分子量
- 28.2 kDa
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