NEW: 请通过本地经销商订购 4A Biotech Co.,Ltd.

HEK-293 Cells Immunoglobulin G4 (IgG4) 同型对照

ABIN2181283 产品详细信息, 供应商: Log in to see
亚型
  • immunoglobulin heavy constant gamma 4 (G4m marker)
  • IGHG4
适用

宿主
HEK-293 Cells
6
1
生物活性
Active
应用范围
Isotype Control (IsoC)
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

亚型 IgG4
产品特性 This protein carries no "tag". The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 33-35 kDa under reducing (R) condition, and 50-55 kDa under non-reducing (NR) condition (SDS-PAGE).
纯度 >95 % as determined by SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
别名 IgG4
背景 Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
分子量 25.8 kDa
UniProt P01861
研究领域 Secondary Antibodies
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
状态 Lyophilized
溶解方式 Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 50 mM Tris, 100 mM Glycine, pH 7.5
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
厂商提供的图像
SDS-PAGE (SDS) image for HEK-293 Cells Immunoglobulin G4 (IgG4) 同型对照 (ABIN2181283) Human IgG4 Fc, Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) condition...
背景 Chen, Jiang, Sun, Han, Wang, Ye, Wang, Zou: "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." in: Journal of proteome research, Vol. 8, Issue 2, pp. 651-61, 2009 (PubMed).

Kristiansen, Bunkenborg, Gronborg, Molina, Thuluvath, Argani, Goggins, Maitra, Pandey: "A proteomic analysis of human bile." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 7, pp. 715-28, 2004 (PubMed).

Frangione, Milstein, Pink: "Structural studies of immunoglobulin G." in: Nature, Vol. 221, Issue 5176, pp. 145-8, 1970 (PubMed).

ODonnell, Frangione, Porter: "The disulphide bonds of the heavy chain of rabbit immunoglobulin G." in: The Biochemical journal, Vol. 116, Issue 2, pp. 261-8, 1970 (PubMed).