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小鼠 IgM 同型对照

ABIN2145425 产品详细信息, 供应商: Log in to see
亚型
宿主
小鼠
95
79
50
13
10
8
6
5
4
4
4
4
3
3
2
2
2
2
1
1
应用范围
Flow Cytometry (FACS), Isotype Control (IsoC)
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亚型 IgM
产品特性 This antibody can be used as a mouse IgM isotype control in flow cytometry and other applications.
纯化方法 Purified
研究领域 Secondary Antibodies
应用备注 Optimal working dilution should be determined by the investigator.
实验流程
  • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
  • Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
  • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
  • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
  • Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
  • Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
  • Repeat step 5.
  • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
  • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
  • [Cell fixation: 2 % formaldehyde solution]
限制 仅限研究用
状态 Liquid
缓冲液 Phosphate-buffered solution, pH 7.4,
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
厂商提供的图像
Flow Cytometry (FACS) image for 小鼠 IgM 同型对照 (ABIN2145425) Mouse IgM isotype control