兔 IgG 同型对照

ABIN1689334 产品详细信息, 供应商: Log in to see
亚型
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克隆类型 (克隆位点)
单克隆 ()
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Isotype Control (IsoC), Negative Control (NC)
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免疫原 The Negative Control is a recombinant rabbit monoclonal antibody against a small molecule, which is normally not present in human or animal.
克隆位点 SP137
亚型 IgG
特异性 The product will not cross-react with human tissues when used at the recommended concentration.
产品特性 Negative Control For Rabbit IgG
纯化方法 Purified by Protein A/G
研究领域 Cardiovascular
应用备注 IHC Procedure: Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
IHC Dilution: 1:400
实验流程 IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100 (2µg/mL). The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
限制 仅限研究用
状态 Liquid
缓冲液 purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide (concentration 200 µg/mL).
储存液 Sodium azide
注意事项 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项 Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
储存条件 4 °C
储存方法 The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
有引用在: Cano, Carmona, Ruiz-Villalba, Rojas, Chau, Wagner, Wagner, Hastie, Muñoz-Chápuli, Pérez-Pomares: "Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 3, pp. 656-61, 2016 (PubMed).

Burdelski, Menan, Tsourlakis, Kluth, Hube-Magg, Melling, Minner, Koop, Graefen, Heinzer, Wittmer, Sauter, Simon, Schlomm, Steurer, Krech: "The prognostic value of SUMO1/Sentrin specific peptidase 1 (SENP1) in prostate cancer is limited to ERG-fusion positive tumors lacking PTEN deletion." in: BMC cancer, Vol. 15, pp. 538, 2015 (PubMed).