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Human IgG 同型对照

ABIN1536459 产品详细信息, 供应商: Log in to see
亚型
宿主
Human
217
207
204
168
160
127
112
93
88
88
88
73
56
50
43
42
27
25
24
22
21
11
8
3
3
2
1
应用范围
Dot Blot (DB), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Isotype Control (IsoC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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亚型 IgG
片段 Whole molecule
产品特性 Purified Human IgG (Whole Molecule) Control is purified from normal human serum through Protein G chromatography. Purified Human IgG (Whole Molecule) Control can be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Western and Dot blots), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reactions.
纯化方法 Protein G chromatography
研究领域 Immunology, Secondary Antibodies
应用备注 Each Investigator should determine their own optimal working dilution for specific applications.
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 4 mg/mL.
浓度 4 mg/mL
缓冲液 PBS, pH 7.4, containing 0.02 % sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing cycles.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
有引用在: van Zeijl, van Koppen, Surovtseva, de Gooyer, Plate, Conti, Karstens, Timmers, Saeed, Wiersinga, Miltenburg, Fliers, Boelen: "Complete inhibition of rhTSH-, Graves' disease IgG-, and M22-induced cAMP production in differentiated orbital fibroblasts by a low-molecular-weight TSHR antagonist." in: The Journal of clinical endocrinology and metabolism, Vol. 97, Issue 5, pp. E781-5, 2012 (PubMed).

Calvert, Kalantarov, Chang, Trakht, Blair, Roehrig: "Human monoclonal antibodies to West Nile virus identify epitopes on the prM protein." in: Virology, Vol. 410, Issue 1, pp. 30-7, 2011 (PubMed).