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小鼠 IgG isotype control (His tag)

ABIN1098612 产品详细信息, 供应商: Log in to see
亚型
宿主
小鼠
217
206
204
169
160
127
112
93
88
88
88
73
56
50
43
42
27
25
24
22
21
11
8
3
3
2
1
抗体类型
Recombinant
标记
His tag
206
169
167
166
154
108
58
58
48
44
19
13
12
12
11
9
9
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Isotype Control (IsoC), SDS-PAGE (SDS)
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亚型 IgG
纯化方法 purified by using conventional chromatography.
纯度 > 90 % by SDS-PAGE
背景 Immunoglobulin G (IgG) is antibody molecules. Each IgG is composed of four peptide chains - two heavy chains gamma and two light chains. Each IgG has two antigen binding sites. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. It also plays an important role in Antibody-dependent cell-mediated cytotoxicity(ADCC) and Intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. Recombinant mouse IgG protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.
Synonyms: Immunoglobulin heavy chain constant region gamma 2a, Immunoglobulin G
分子量 27.5 kDa (242aa)
研究领域 Immunology, Secondary Antibodies
应用备注 Each Investigator should determine their own optimal working dilution for specific applications.
说明

Concentration (detection method): 1 mg/mL (determined by Bradford assay)

限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 20 mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol
注意事项 Avoid repeated freezing and thawing cycles.
储存条件 4 °C
厂商提供的图像
 image for 小鼠 IgG isotype control (His tag) (ABIN1098612) Mouse IgG isotype control (His tag)
背景 Mallery, McEwan, Bidgood, Towers, Johnson, James: "Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 46, pp. 19985-90, 2010 (PubMed).

Stadlmann, Pabst, Kolarich, Kunert, Altmann: "Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides." in: Proteomics, Vol. 8, Issue 14, pp. 2858-71, 2008 (PubMed).