Caspase-8 Inhibitor Z-IETD-FMK

ABIN412376 产品详细信息, 供应商: Log in to see
应用范围
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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原理 A caspase-8 inhibitor
序列 Z-Ile-Glu(OMe)-Thr-Asp(OMe)-FMK
产品特性 PubChem CID: 44135212
A synthetic peptide that irreversibly inhibits FLICE and related protease/caspase activity and blocks apoptosis. IETD is the specific recognition sequence found in CPP32 proenzyme. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups.)
纯度 > 99 % by HPLC
Chemical Name Z-IETD-FMK, Caspase-8 Inhibitor, Z-IETD-fluoromethylketone
Formula C₃₀H₄₃FN₄O₁₁
Permeability Cell-permeable
Solubility DMSO
分子量 654.7 g/mol
应用备注 We recommend using 5000X dilutions for inhibiting caspase-8 activity in Jurkat cell culture (e.g., Add 1 l to 5 mL of culture medium). The optimal doses may vary for different cells and culture conditions.
限制 仅限研究用
状态 Liquid
浓度 10 mM
注意事项 Protect from light and moisture
储存条件 -20 °C
有效期 12 months
厂商提供的图像
 image for Caspase-8 Inhibitor Z-IETD-FMK (ABIN412376) Caspase-8 Inhibitor Z-IETD-FMK
有引用在: Westphal, Sytnyk, Schachner, Leshchynska: "Clustering of the neural cell adhesion molecule (NCAM) at the neuronal cell surface induces caspase-8- and -3-dependent changes of the spectrin meshwork required for NCAM-mediated neurite outgrowth." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 42046-57, 2010 (PubMed).

Tang, Liu, Wang, Wang, Cao, Mi, Zhang: "Involvement of caspase 8 in apoptosis induced by ultrasound-activated hematoporphyrin in sarcoma 180 cells in vitro." in: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine, Vol. 27, Issue 4, pp. 645-56, 2008 (PubMed).

Deng, Yin, Lu, Cai, Yin: "The apoptotic effect of brucine from the seed of Strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 91, Issue 1, pp. 59-69, 2006 (PubMed).

Teachey, Obzut, Axsom, Choi, Goldsmith, Hall, Hulitt, Manno, Maris, Rhodin, Sullivan, Brown, Grupp: "Rapamycin improves lymphoproliferative disease in murine autoimmune lymphoproliferative syndrome (ALPS)." in: Blood, Vol. 108, Issue 6, pp. 1965-71, 2006 (PubMed).

Gillet, Dewals, Farnir, de Leval, Vanderplasschen: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Song, Mahidhara, Zhou, Hoffman, Seol, Flavell, Billiar, Otterbein, Choi: "Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 2, pp. 1220-6, 2004 (PubMed).

Song, Zhou, Kim, Shapiro, Liu, Ferran, Choi, Otterbein: "Carbon monoxide promotes Fas/CD95-induced apoptosis in Jurkat cells." in: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 44327-34, 2004 (PubMed).

Wang, Grégori, Hullinger, Andrisani et al.: "Sustained activation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase pathways by hepatitis B virus X protein mediates apoptosis via induction of Fas/FasL and tumor necrosis factor ..." in: Molecular and cellular biology, Vol. 24, Issue 23, pp. 10352-65, 2004 (PubMed).

Yen, Mar, Craciunescu, Edwards, Zeisel: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).

Frost, Delikat, Al-Mehairi, Sinclair: "Regulation of p27KIP1 in Epstein-Barr virus-immortalized lymphoblastoid cell lines involves non-apoptotic caspase cleavage." in: The Journal of general virology, Vol. 82, Issue Pt 12, pp. 3057-66, 2001 (PubMed).