anti-人 ERN1 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Recommended ERN1 Antibody (supplied by: Log in to see )

抗原
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) 抗体
  • 9030414B18Rik
  • AI225830
  • C85377
  • Ire1a
  • Ire1alpha
  • Ire1p
  • Ern1
  • Ire1
  • Ire1b
  • IRE1
  • IRE1P
  • IRE1a
  • hIRE1p
  • RGD1559716
  • endoplasmic reticulum (ER) to nucleus signalling 1
  • endoplasmic reticulum (ER) to nucleus signalling 2
  • endoplasmic reticulum to nucleus signaling 1
  • endoplasmic reticulum to nucleus signalling 1
  • Ern1
  • Ern2
  • ERN1
  • LOC100357346
抗原表位
AA 700-800
33
20
18
18
15
15
15
15
14
10
8
8
8
8
7
6
4
4
2
1
1
1
1
适用
人, 小鼠, 大鼠
190
107
74
13
13
13
12
12
2
1
1
宿主

158
32
16
3
克隆类型
多克隆
标记
This ERN1 antibody is un-conjugated
13
12
11
6
6
6
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
176
97
43
33
32
31
26
14
8
5
4
1
1
Supplier
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产品编号 ABIN250711
产品在该地区无法提供。
获取报价
中国 北京 101111
No. 88 KeChuang 6th Street
Room 801-803
Beijing Economic Technological Development Area
4A Biotech Co.,Ltd.
Tel 400-7060-959
传真 +86 10 56315212-8813
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.400806 ABIN4961671 IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN250706 ELISA ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit pSer724 Log in to see Polyclonal 140
1 ABIN1387531 IF (p) IHC (p) WB Rabbit IgG AA 700-750, pSer726 Log in to see Polyclonal 2
1 ABIN250707 ELISA ICC IF IHC (fro) IHC (p) WB Biotin Rabbit pSer724 Log in to see Polyclonal 2
1 ABIN1999391 ELISA IHC (p) Mouse IgG2b AA 465-977 Log in to see 10B2B2 3
1 ABIN250708 ELISA IHC (fro) IHC (p) WB HRP Rabbit pSer724 Log in to see Polyclonal 2
1 ABIN1387555 IF (p) IHC (p) WB Rabbit IgG AA 250-300 Log in to see Polyclonal 5
1 ABIN1107134 EIA IHC (p) WB Mouse IgG1 AA 282-433 Log in to see 9F2 2
1 ABIN781867 EIA IHC (p) WB Mouse IgG1 Log in to see 3D1
1 ABIN574080 FACS IF IHC (p) ELISA WB Mouse IgG1 AA 282-433 Log in to see 9F2
1 ABIN626045 IHC (p) ELISA WB Mouse IgG1, kappa AA 401-500 Log in to see 3D1
1 ABIN1101382 ICC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1418014 IHC (p) WB HRP Rabbit IgG AA 250-300 Log in to see Polyclonal
1 ABIN4327529 ICC IF IHC IHC (p) WB DyLight 405 Rabbit AA 700-800 Log in to see Polyclonal
1 ABIN4327534 ICC IF IHC IHC (p) WB Alexa Fluor 405 Rabbit AA 700-800 Log in to see Polyclonal
1 ABIN4327535 ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 700-800 Log in to see Polyclonal
1 ABIN4327536 ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 700-800 Log in to see Polyclonal
1 ABIN4327537 ICC IF IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 700-800 Log in to see Polyclonal
1 ABIN4327524 ICC IF IHC IHC (p) WB DyLight 650 Rabbit AA 700-800 Log in to see Polyclonal
1 ABIN4327525 ICC IF IHC IHC (p) WB DyLight 550 Rabbit AA 700-800 Log in to see Polyclonal

引用最多的anti-ERN1 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-ERN1 抗体

应用范围 / 适用 大鼠 小鼠
ELISA 27 抗体 39 抗体
Enzyme Immunoassay (EIA) 2 抗体 2 抗体
Immunocytochemistry (ICC) 33 抗体 33 抗体
Immunofluorescence (IF) 25 抗体 25 抗体
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 抗体 13 抗体
Immunohistochemistry (IHC) 16 抗体 16 抗体
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 抗体 14 抗体
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 抗体 29 抗体
Immunoprecipitation (IP) 7 抗体 7 抗体
Western Blotting (WB) 61 抗体 93 抗体

General

抗原 Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) 抗体
抗原表位 AA 700-800
(33), (20), (18), (18), (15), (15), (15), (15), (14), (10), (8), (8), (8), (8), (7), (6), (4), (4), (2), (1), (1), (1), (1)
适用 人, 小鼠, 大鼠
(190), (107), (74), (13), (13), (13), (12), (12), (2), (1), (1)
宿主
(158), (32), (16), (3)
克隆类型
多克隆
标记 This ERN1 antibody is un-conjugated
(13), (12), (11), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
应用范围 Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(176), (97), (43), (33), (32), (31), (26), (14), (8), (5), (4), (1), (1)
Pubmed 17个引用可参考
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ERN1抗体详情

ERN1目标详情 使用细节 贮存及处理 ProductDetails: References for anti-ERN1 antibody (ABIN250711) 图像
特异性 This antibody detects the levels of endogenous total IRE1 Alpha protein.
纯化方法 Immunogen affinity purified
免疫原 A synthetic peptide within the human IRE1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]

ERN1目标详情

ERN1抗体详情 使用细节 贮存及处理 ProductDetails: References for anti-ERN1 antibody (ABIN250711) 图像 回页首
抗原
别名 IRE1 alpha (ERN1 Antibody 摘要)
背景 Gene Symbol: ERN1
分子量 Theoretical MW: 110 kDa
基因ID 2081
UniProt O75460
途径 ER-Nucleus Signaling

使用细节

ERN1抗体详情 ERN1目标详情 贮存及处理 ProductDetails: References for anti-ERN1 antibody (ABIN250711) 图像 回页首
应用备注 Western Blot 1:1000-1:2000, Immunohistochemistry 1:250-1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:250, Immunohistochemistry-Paraffin 1:250-1:500This IRE1 alpha antibody is useful for Western blot, where a band ~110 kDa is observed. In ICC/IF endoplasmic reticulum staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

实验流程 ICC/IF Protocol for IRE1 alpha Antibody Immunocytochemistry ProtocolCulture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 5-10 minutes.
. Remove the formalin and add 0.5 % Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1 % Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
. To block nonspecific antibody binding incubate in 10 % normal goat serum for 1 hour at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 µg/ml, or coverslips can be directly mounted in media containing DAPI.
. Cells can now be viewed with a fluorescence microscope.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.
限制 仅限研究用

贮存及处理

ERN1抗体详情 ERN1目标详情 使用细节 ProductDetails: References for anti-ERN1 antibody (ABIN250711) 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze-thaw cycles
储存条件 -20 °C
储存方法 Store at -20°C. Avoid freeze-thaw cycles.

ProductDetails: References for anti-ERN1 antibody (ABIN250711)

ERN1抗体详情 ERN1目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Alnasser, Guan, Zhang, Gleave, Nguan, Du: "Requirement of clusterin expression for prosurvival autophagy in hypoxic kidney tubular epithelial cells." in: American journal of physiology. Renal physiology, Vol. 310, Issue 2, pp. F160-73, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Kim, Jun, Lee: "Effect of Cudrania tricuspidata and Kaempferol in Endoplasmic Reticulum Stress-Induced Inflammation and Hepatic Insulin Resistance in HepG2 Cells." in: Nutrients, Vol. 8, Issue 1, 2016 (Sample species: Human). Further details: Western Blotting

Vannuvel, Van Steenbrugge, Demazy, Ninane, Fattaccioli, Fransolet, Renard, Raes, Arnould: "Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1913-31, 2016 (Sample species: Human). Further details: Western Blotting

Li, Zalzala, Jadhav, Xu, Kasumov, Yin, Zhang: "Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, endoplasmic reticulum stress, and lipogenesis and is regulated by hepatocyte nuclear factor 4 alpha in mice." in: Hepatology (Baltimore, Md.), Vol. 63, Issue 6, pp. 1860-74, 2016

Tripathi, Zhang, Singh, Sinha, Moe, DeSilva, Yen: "Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation." in: Cell death & disease, Vol. 7, Issue 12, pp. e2513, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Ghosh, Garg, Mau, OBrien, Liu, Yung: "Elevated Endoplasmic Reticulum Stress Response Contributes to Adipose Tissue Inflammation in Aging." in: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 70, Issue 11, pp. 1320-9, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Clarke, Lyra E Silva, Figueiredo, Frozza, Ledo, Beckman, Katashima, Razolli, Carvalho, Frazão, Silveira, Ribeiro, Bomfim, Neves, Klein, Medeiros, LaFerla, Carvalheira, Saad, Munoz, Velloso, Ferreira et al.: "Alzheimer-associated Aβ oligomers impact the central nervous system to induce peripheral metabolic deregulation. ..." in: EMBO molecular medicine, Vol. 7, Issue 2, pp. 190-210, 2015 Further details: Western Blotting

Quan, Wang, Liang, Zheng, Zhang: "Melatonin inhibits tunicamycin-induced endoplasmic reticulum stress and insulin resistance in skeletal muscle cells." in: Biochemical and biophysical research communications, Vol. 463, Issue 4, pp. 1102-7, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Liu, Fan, Tang, Ke: "The resveratrol attenuates ethanol-induced hepatocyte apoptosis via inhibiting ER-related caspase-12 activation and PDE activity in vitro." in: Alcoholism, clinical and experimental research, Vol. 38, Issue 3, pp. 683-93, 2014 (Sample species: Human). Further details: Western Blotting

Wu, Yang, Fan, Peng, Deng, Ding, Yang, Zhou, Miao, Fu: "Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis." in: Cell death & disease, Vol. 5, pp. e1464, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Zheng, Zhang, Joe, Kim, Uddin, Rhew, Kim, Ryter, Chung: "Carbon monoxide-releasing molecules reverse leptin resistance induced by endoplasmic reticulum stress." in: American journal of physiology. Endocrinology and metabolism, Vol. 304, Issue 7, pp. E780-8, 2013 Further details: Western Blotting

Arshad, Ye, Gu, Wong, Liu, Li, Zhou, Zhang, Bay, Yu, Li: "RNF13, a RING finger protein, mediates endoplasmic reticulum stress-induced apoptosis through the inositol-requiring enzyme (IRE1α)/c-Jun NH2-terminal kinase pathway." in: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8726-36, 2013 (Sample species: Human). Further details: Western Blotting

Kurata, Yamazaki, Kanno, Ishibashi, Takahara, Kitagawa, Nakamura: "Anti-apoptotic function of Xbp1 as an IL-3 signaling molecule in hematopoietic cells." in: Cell death & disease, Vol. 2, pp. e118, 2011 (Sample species: Mouse (Murine)). Further details: Western Blotting

Luo, He, Zhang, Yu, Chen, Xu, Tang, Urano, Min: "AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response." in: The Journal of biological chemistry, Vol. 283, Issue 18, pp. 11905-12, 2008 (Sample species: Mouse (Murine)). Further details: Western Blotting

Lipson, Fonseca, Ishigaki, Nguyen, Foss, Bortell, Rossini, Urano: "Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1." in: Cell metabolism, Vol. 4, Issue 3, pp. 245-54, 2006 (Sample species: Mouse (Murine)). Further details: Western Blotting

Fonseca, Fukuma, Lipson, Nguyen, Allen, Oka, Urano: "WFS1 is a novel component of the unfolded protein response and maintains homeostasis of the endoplasmic reticulum in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 280, Issue 47, pp. 39609-15, 2005

Kubota, Lee, Tsuchiya, Young, Everett, Martinez-Mier, Snead, Nguyen, Urano, Bartlett: "Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 23194-202, 2005

图像

ERN1抗体详情 ERN1目标详情 使用细节 贮存及处理 ProductDetails: References for anti-ERN1 antibody (ABIN250711) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-ERN1 抗体 (Endoplasmic Reticulum To Nucleus Signaling 1) (AA 700-800) (ABIN250711) Detection of Ire1 alpha in transfected Min6 cells using ABIN250711.
Western Blotting (WB) image for anti-ERN1 抗体 (Endoplasmic Reticulum To Nucleus Signaling 1) (AA 700-800) (ABIN250711) Western Blot: IRE1 alpha Antibody [ABIN250711] - Western Blot detection of Total IRE1...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERN1 抗体 (Endoplasmic Reticulum To Nucleus Signaling 1) (AA 700-800) (ABIN250711) Immunohistochemistry-Paraffin: IRE1 alpha Antibody [ABIN250711] - IHC-P analysis of a...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERN1 抗体 (Endoplasmic Reticulum To Nucleus Signaling 1) (AA 700-800) (ABIN250711) Immunohistochemistry-Paraffin: IRE1 alpha Antibody [ABIN250711] - IHC-P analysis of a...
Immunofluorescence (IF) image for anti-ERN1 抗体 (Endoplasmic Reticulum To Nucleus Signaling 1) (AA 700-800) (ABIN250711) Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody [ABIN250711] - The IRE-1 ...