小鼠 EIF2AK2 ELISA Kit

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抗原
Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) ELISA试剂盒
  • PKR
  • pkr
  • EIF2AK2
  • DKFZp469K1934
  • EIF2AK1
  • PRKR
  • 2310047A08Rik
  • 4732414G15Rik
  • AI467567
  • AI747578
  • Pkr
  • Prkr
  • Tik
  • eukaryotic translation initiation factor 2-alpha kinase 2
  • EIF2AK2
  • eif2ak2
  • Eif2ak2
适用
小鼠
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Kits with alternative reactivity to:
13
4
实验类型
Competition ELISA
应用范围
ELISA
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产品编号 ABIN454366
3,481.66 ¥
(含税, 含运费)
来自4A Biotech Co.,Ltd.(本地经销商)
获取报价
中国 北京 101111
No. 88 KeChuang 6th Street
Room 801-803
Beijing Economic Technological Development Area
4A Biotech Co.,Ltd.
Tel 400-7060-959
传真 +86 10 56315212-8813

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应用范围 / 适用 大鼠
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General

抗原 Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) ELISA试剂盒
适用 小鼠
Kits with alternative reactivity to:
(13), (4)
实验类型 Competition ELISA
应用范围 ELISA
Supplier Log in to see

EIF2AK2ELISA Kit详情

目标详细情况 使用细节 贮存及处理 图像
原理 This immunoassay kit allows for the in vitro quantitative determination of mouse Anti-nuclear Antibody, ANA concentrations in cell culture supernates, serum, plasma and other biological fluids.
样品类型 Cell Culture Supernatant, Serum, Plasma, Biological Fluids
检测方法 Colorimetric
Analytical Method Quantitative
特异性 This assay recognizes recombinant and natural mouse ANA.
交叉反应 (详细) No significant cross-reactivity or interference was observed.
灵敏度 < 0.78 U/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
产品特性 Mus musculus,Mouse,Interferon-induced, double-stranded RNA-activated protein kinase,Eukaryotic translation initiation factor 2-alpha kinase 2,eIF-2A protein kinase 2,Interferon-inducible RNA-dependent

目标详细情况

EIF2AK2ELISA Kit详情 使用细节 贮存及处理 图像 回页首
抗原
别名 Eif2ak2 (EIF2AK2 ELISA Kit 摘要)
物质类 Antibody
背景 Anti-nuclear antibodies (ANAs, also known as anti-nuclear factor or ANF) are antibodies directed against contents of the cell nucleus.They are present in higher than normal numbers in autoimmune disease. The ANA test measures the pattern and amount of autoantibody which can attack the body's tissues as if they were foreign material. Autoantibodies are present in low titers in the general population, but in about 5% of the population, their concentration is increased, and about half of this 5% have an autoimmune disease. Normal titer of ANA is 1:40. Higher titers are indicative of an autoimmune disease. The presence of ANA is indicative of lupus erythematosus (present in 80-90% of cases), though they also appear in some other auto-immune diseases such as Sj ö gren's syndrome (60%), rheumatoid arthritis, autoimmune hepatitis, scleroderma and polymyositis & dermatomyositis (30%), and various non-rheumatological conditions associated with tissue damage. ANA are also directed to the nuclear pore complex in primary biliary cirrhosis. Other conditions with high ANA titre include Addison disease, Idiopathic thrombocytopenic purpura (ITP), Hashimoto's, Autoimmune hemolytic anemia, Type I diabetes mellitus, Mixed connective tissue disorder.
途径 ER-Nucleus Signaling, Hepatitis C

使用细节

EIF2AK2ELISA Kit详情 目标详细情况 贮存及处理 图像 回页首
样本量 100 μL
板类型 Pre-coated
实验流程 The microtiter plate provided in this kit has been pre-coated with an antigen specific to ANA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen preparation specific for ANA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ANA, biotin-conjugated antigen and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANA in the samples is then determined by comparing the O.D. 2 of the samples to the standard curve.
限制 仅限研究用

贮存及处理

EIF2AK2ELISA Kit详情 目标详细情况 使用细节 图像 回页首
储存条件 4 °C/-20 °C
储存方法 The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.