anti-小鼠 EIF2AK2 抗体 for Immunofluorescence (Paraffin-embedded Sections)

Recommended EIF2AK2 Antibody (supplied by: Log in to see )

抗原
Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) 抗体
  • PKR
  • pkr
  • EIF2AK2
  • DKFZp469K1934
  • EIF2AK1
  • PRKR
  • 2310047A08Rik
  • 4732414G15Rik
  • AI467567
  • AI747578
  • Pkr
  • Prkr
  • Tik
  • eukaryotic translation initiation factor 2 alpha kinase 2
  • eukaryotic translation initiation factor 2-alpha kinase 2
  • EIF2AK2
  • eif2ak2
  • Eif2ak2
抗原表位
AA 260-310
61
54
28
19
18
15
15
13
11
6
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠, 大鼠
291
78
42
2
2
2
2
1
1
1
1
宿主

248
47
克隆类型
多克隆
标记
This EIF2AK2 antibody is un-conjugated
7
7
6
5
5
4
4
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
190
128
99
80
48
44
19
16
4
4
2
1
1
选项
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产品编号 ABIN687307
$ 318.45
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN687311 IF (p) Cy5 Rabbit IgG AA 260-310 Log in to see Polyclonal
1 ABIN687310 IF (p) Cy3 Rabbit IgG AA 260-310 Log in to see Polyclonal
1 ABIN687312 IF (p) Cy5.5 Rabbit IgG AA 260-310 Log in to see Polyclonal
1 ABIN687313 IF (p) Cy7 Rabbit IgG AA 260-310 Log in to see Polyclonal
1 ABIN687314 IF (p) FITC Rabbit IgG AA 260-310 Log in to see Polyclonal
1 ABIN895063 IF (p) Alexa Fluor 350 Rabbit IgG AA 260-310 Log in to see Polyclonal
1 ABIN895065 IF (p) Alexa Fluor 488 Rabbit IgG AA 260-310 Log in to see Polyclonal
1 ABIN895064 IF (p) Alexa Fluor 555 Rabbit IgG AA 260-310 Log in to see Polyclonal
1 ABIN895066 IF (p) Alexa Fluor 647 Rabbit IgG AA 260-310 Log in to see Polyclonal

General

抗原 Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) 抗体
抗原表位 AA 260-310
(61), (54), (28), (19), (18), (15), (15), (13), (11), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠, 大鼠
(291), (78), (42), (2), (2), (2), (2), (1), (1), (1), (1)
宿主
(248), (47)
克隆类型
多克隆
标记 This EIF2AK2 antibody is un-conjugated
(7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(190), (128), (99), (80), (48), (44), (19), (16), (4), (4), (2), (1), (1)
Pubmed 找到1个引用
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EIF2AK2抗体详情

EIF2AK2目标详情 使用细节 贮存及处理 References for anti-EIF2AK2 antibody (ABIN687307) 图像
纯化方法 Purified by Protein A.
免疫原 KLH conjugated synthetic peptide derived from human PKR
亚型 IgG

EIF2AK2目标详情

EIF2AK2抗体详情 使用细节 贮存及处理 References for anti-EIF2AK2 antibody (ABIN687307) 图像 回页首
抗原
别名 Eif2ak2/Pkr (EIF2AK2 Antibody 摘要)
背景

IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2).

Subcellular location: Cytoplasm, Nucleus

Synonyms: Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, Protein kinase RNA-activated, PKR, Tyrosine-protein kinase EIF2AK2, p68 kinase, EIF2AK2, PRKR

基因ID 5610
UniProt P19525
途径 ER-Nucleus Signaling, Hepatitis C

使用细节

EIF2AK2抗体详情 EIF2AK2目标详情 贮存及处理 References for anti-EIF2AK2 antibody (ABIN687307) 图像 回页首
应用备注 WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
限制 仅限研究用

贮存及处理

EIF2AK2抗体详情 EIF2AK2目标详情 使用细节 References for anti-EIF2AK2 antibody (ABIN687307) 图像 回页首
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20°C
有效期 12 months

References for anti-EIF2AK2 antibody (ABIN687307)

EIF2AK2抗体详情 EIF2AK2目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Lopušná, Benkóczka, Lupták, Matúšková, Lukáčiková, Ovečková, Režuchová: "Murine gammaherpesvirus targets type I IFN receptor but not type III IFN receptor early in infection." in: Cytokine, Vol. 83, pp. 158-70, 2016

图像

EIF2AK2抗体详情 EIF2AK2目标详情 使用细节 贮存及处理 References for anti-EIF2AK2 antibody (ABIN687307) 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 260-310) antibody (ABIN687307) Formalin-fixed and paraffin embedded rat kidney labeled with Anti EIF2AK2/PKR Polyclo...
Immunohistochemistry (IHC) image for anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 260-310) antibody (ABIN687307) Formalin-fixed and paraffin embedded rat brain labeled with Anti-EIF2AK2/PKR Polyclon...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 260-310) antibody (ABIN687307) Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue, Antigen retrieval by b...