β-Glucosyltransferase enzyme

ABIN2669404 产品详细信息, 供应商: Log in to see
抗原
适用
Bacteriophage T4
宿主
大肠杆菌(E. Coli)
应用范围
Activity Assay (AcA), Tet-assisted Bisulfite Sequencing (TAB-Seq)
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产品特性 β-Glucosyltransferase will transfer the glucose moiety from the UDP-Glucose (uridine diphosphoglucose) to 5-hydroxymethylcytosine residues in double-stranded DNA.
组件 200,000 Units β-Glucosyltransferase enzyme
10X β-Glucosyltransferase Reaction Buffer
50 mM UDP-Glucose
1 M DTT
应用备注 The β-Glucosyltransferase enzyme can be used to glucosylate 5-hmC DNA. Glucosylated DNA can be used as a differentiator with glucosyl-sensitive restriction enzymes. The use of a labeled [14C] UDP-Glucose donor would enable labeling of the 5-hmC residues.
说明

One unit is defined as the amount of enzyme required to fully protect 0.5 µg of 5-hmC DNA Standard (Catalog No. 55008) in 1 hour at 37°C in a total reaction volume of 50 µl from cleavage by 4 units of GlaI restriction enzyme in an incubation of 16 hours at 30°C. A pilot titration of enzyme is recommended for protection of genomic DNA.

限制 仅限研究用
储存条件 -20 °C/-80 °C
储存方法 Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
厂商提供的图像
Restriction Digest (RD) image for β-Glucosyltransferase enzyme (ABIN2669404) Figure 1: 0.5 µg of a 5-hmC Methylated DNA Standard (Catalog No. 55008) was incubated...